Question

in eukaryotic dna replication is there 1 replication fork and is there where dna replication starts

how about bacteria dna replication is there 1 replication fork and what does it mean when baxterial dna replictes at the dna

what are the structural and molecular differences between eukaryotic and bacterial dna replication

Answer 1

In eukaryotic DNA replication there are multiple origins of replication and replication starts at multiple point making multiple replication forks. However the replication is unidirectional.

In Bacterial DNA replication, there is single point of origin and a single replication fork. The bacterial DNA replication occurs in both the direction by forming Okazaki fragment in the 3'-5' direction template.

There are certain differences between eukaryotic and bacterial or prokaryotic DNA replication.

Structurally,

1. Bacterial replication is process for the replication of circular and double stranded DNA while eukaryotic DNA replication occurs for the linear and double stranded DNA.

2. There is single origin of replication in bacteria while in eukaryotes there are multiple origin of replication in a single chromosome.

3. During bacterial DNA reflection the Okazaki fragments are large while in eukaryotes, the Okazaki fragments are very small.

4. After the replication is completed, there is formation of 2 circular chromosomes in bacterial replication while in eukaryotic replication the end product is the two sister chromatids.

In molecular,

1. Bacterial replication involves DNA polymerase i and III while in eukaryotic replication DNA polymerase $\alpha$ , $\beta$ and $\varepsilon$ are involved.

2. In Bacterial replication DNA gyrase is required while in eukaryotic replication DNA gyrase is not required.