If you have a culture of E. coli at a concentration of 5x10bacteria/mL and you infect...

Question

Question

Answer 1

Answer #1

The concentration of phage should be 1.5 X 10⁸.

The multiplication of infection can be calculated as

MOI = Concentration of plaques forming phage ÷ Total number of host cells

Putting the values, we have

3 = Concentration of phage ÷ 5 X 10⁷

Or, concentration of phage = 3 X 5 X 10⁷ = 15 X 10^7.

Answer 2

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