Homework Answers
a) 1.
The enzyme Dde I, has a restriction site 5'-CCCGGG-3'. The DNA sequence doesn't contain this restriction site. So, there will be only one fragment, that is the native fragment of the DNA.
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CHAPTER 14: Using the restriction enzyme indicated, predict HOW MANY fragments will result after using that...
UGLIOLI 0. PLS You will be using the restriction enzyme Haelll to cut your DNA. What...
UGLIOLI 0. PLS You will be using the restriction enzyme Haelll to cut your DNA. What is the sequence that Haelll recognizes? (see link in prelab module for information on specific restriction enzymes). < 5'...AGCT... 3' 3' ...IC GA... 5' B 5' ...GG c... 3' 3' ...CC GG... 5' 0 5' ...G GALCC... 3' 3' ...CCI AGG... 5' 5 ...A AGCIL... 3' 3 ...ITCGA A... 5' D E S ALES 0 0 0 0 0.5 pts
Number 3 LS7A Problem Solving Session 8 (Week 9) 1. Using the space below describe the...
Number 3
LS7A Problem Solving Session 8 (Week 9) 1. Using the space below describe the function of a restriction enzyme using the terms double stranded break, cut site, palindrome, sticky ends, and blunt ends. Explain your description to your LA or neighbor. 2. Draw all the possible fragments generated from the following restriction enzyme cut sites indicated ("*") on the double stranded DNA below. 3- AGCCCT-5 5--TCGC-GA-3' 3. Circle the correct fragments that can be generated from the restriction...
Number 1 and 2. LS7A Problem Solving Session 8 (Week 9) 1. Using the space below...
Number 1 and 2.
LS7A Problem Solving Session 8 (Week 9) 1. Using the space below describe the function of a restriction enzyme using the terms double stranded break, cut site, palindrome, sticky ends, and blunt ends. Explain your description to your LA or neighbor. 2. Draw all the possible fragments generated from the following restriction enzyme cut sites indicated ("*") on the double stranded DNA below. 3- AGCCCT-5 5--TCGC-GA-3' 3. Circle the correct fragments that can be generated from...
please answer question numbe 2. Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
on the me but not with the larger fragments on o t and more and record...
on the me but not with the larger fragments on o t and more and record med in the h 3. Determine the distracted for each bund data bere 4. Determine the distance traveled for each and generated in the double digest and record data bere tion Endonuclease Digestion and Gel Electrophoresis of DNA 185 VI. RESTRICTION MAPPING The different DNA fragments generated by different map DNA. For example, specific DNA on 2000 nucleotides in las Rl or Hind and...
colony which one express gene Only correct explanation not just answears pls. how to determine correct...
colony which one express gene
Only correct explanation not just answears pls.
how to determine correct orientation by electrophoresis
CORI -Hindi mal kpn ! Aggi -Hind III Gene CDNA EcoRI 5. G' AAT TC 3 CTTAAG. 5 Бра 5GGT ACC 3 3 CCATGG 5 pMAE2 Avr 11 M5 CCTAGGS 3 GGATCC.5 Arell ACCGGT 3 3.. TGGCCA...5 Amp Hind 1 5: AAGCTT 3 S 3. TTCGAA53 Xma C CGGG.3 GGGCGC 5 You try the following strategies in order to see if...
5. If you are digesting a large linear fragment that is 300,000 bp in length. How...
5. If you are digesting a large linear fragment that is 300,000 bp in length. How many fragments would be produced if the DNA was digested by the following enzymes? Assume the fragment sizes are the average sizes calculated for a random DNA sequence. Use the information in the table from Question 3. Show your work. (A) Smal (B) Haelll (C) Noti Table 4.1 Recognition Sequences and Cutting Sites of Selected Restriction Endonucleases Enzyme Alul BamHI Bgli Clal ECORI Haelll...
i need from a to i please step by step Question 2 (50 points) The template...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
UGLIOLI 0. PLS You will be using the restriction enzyme Haelll to cut your DNA. What is the sequence that Haelll recognizes? (see link in prelab module for information on specific restriction enzymes). < 5'...AGCT... 3' 3' ...IC GA... 5' B 5' ...GG c... 3' 3' ...CC GG... 5' 0 5' ...G GALCC... 3' 3' ...CCI AGG... 5' 5 ...A AGCIL... 3' 3 ...ITCGA A... 5' D E S ALES 0 0 0 0 0.5 pts
Number 3
LS7A Problem Solving Session 8 (Week 9) 1. Using the space below describe the function of a restriction enzyme using the terms double stranded break, cut site, palindrome, sticky ends, and blunt ends. Explain your description to your LA or neighbor. 2. Draw all the possible fragments generated from the following restriction enzyme cut sites indicated ("*") on the double stranded DNA below. 3- AGCCCT-5 5--TCGC-GA-3' 3. Circle the correct fragments that can be generated from the restriction...
Number 1 and 2.
LS7A Problem Solving Session 8 (Week 9) 1. Using the space below describe the function of a restriction enzyme using the terms double stranded break, cut site, palindrome, sticky ends, and blunt ends. Explain your description to your LA or neighbor. 2. Draw all the possible fragments generated from the following restriction enzyme cut sites indicated ("*") on the double stranded DNA below. 3- AGCCCT-5 5--TCGC-GA-3' 3. Circle the correct fragments that can be generated from...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
on the me but not with the larger fragments on o t and more and record med in the h 3. Determine the distracted for each bund data bere 4. Determine the distance traveled for each and generated in the double digest and record data bere tion Endonuclease Digestion and Gel Electrophoresis of DNA 185 VI. RESTRICTION MAPPING The different DNA fragments generated by different map DNA. For example, specific DNA on 2000 nucleotides in las Rl or Hind and...
colony which one express gene
Only correct explanation not just answears pls.
how to determine correct orientation by electrophoresis
CORI -Hindi mal kpn ! Aggi -Hind III Gene CDNA EcoRI 5. G' AAT TC 3 CTTAAG. 5 Бра 5GGT ACC 3 3 CCATGG 5 pMAE2 Avr 11 M5 CCTAGGS 3 GGATCC.5 Arell ACCGGT 3 3.. TGGCCA...5 Amp Hind 1 5: AAGCTT 3 S 3. TTCGAA53 Xma C CGGG.3 GGGCGC 5 You try the following strategies in order to see if...
5. If you are digesting a large linear fragment that is 300,000 bp in length. How many fragments would be produced if the DNA was digested by the following enzymes? Assume the fragment sizes are the average sizes calculated for a random DNA sequence. Use the information in the table from Question 3. Show your work. (A) Smal (B) Haelll (C) Noti Table 4.1 Recognition Sequences and Cutting Sites of Selected Restriction Endonucleases Enzyme Alul BamHI Bgli Clal ECORI Haelll...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
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