Restriction endonucleases.... a) are used to cut RNA at defined sequences. b) can be used to...
Restriction endonucleases....
a) are used to cut RNA at defined sequences.
b) can be used to create pieces of DNA with cohesive ends.
c) have no specific sequence requirements for recognition or cutting.
d) were identified as a protozoan defense mechanism against viruses.
e) are used in standard DNA sequencing reactions.
Homework Answers
Restriction endonucleases are used to make slice of DNA with cohesive ends ( option is B). There are different types of restriction endonuclease or enzyme which cut the DNA into so many pieces. This enzyme is basically found in Prokaryotes defense mechanism to cutting the viral DNA and avoiding virus infection. But nowadays, modified restoration enzymes are used to cutting DNA in Biotechnology and improve the Biological Sciences. One restriction endonuclease can first recognized the DNA sequences and converted the DNA into pieces to cutting the specific site of the DNA.
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Restriction endonucleases....
a) are used to cut RNA at defined sequences.
b) can be used to...
find the errors Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at...
find the errors
Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at specific locations at the target sequence. The result of digesting a particular genome with a particular restriction enzyme is a collection of restriction fragments of defined length and composition. These can be used to generate restriction maps or create pieces with sticky ends. These sticky ends can be used to attach to other fragments that have sticky ends caused by cutting with a different...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a bacteria defense mechanism c. They can leave single-stranded overhanging sequences called Sticky ends d. They recognize a specific target sequence e.They cut DNA
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
Restriction endonucleases are: [ can be more than one] Scientists received Nobel Prize in 1978 for...
Restriction endonucleases are: [ can be more than one] Scientists received Nobel Prize in 1978 for discovering restriction endonucleases. Bacteria keeps their own DNA modified preventing digestion by their own enzyme. Enzymes found in bacteria that digests DNA. Each restriction enzyme recognizes a specific DNA sequence. Bacteria uses their own restriction enzymes for their defense against viral infection. Which of the following is/are correct for chemical synthesis of oligo DNA? answer can be more than one Any nucleotide is added...
please answer question numbe 2. Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a)...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The reaction is specific for certain sequences in the DNA. 2) Only small amounts of template are needed. 3) Results can only be obtained with freshly isolated DNA. 4) All the products from a specific part of the DNA will be the same size. Question 2: Saving The chain terminator used in Sanger's DNA sequencing is works due to.. 1) lack of 2'OH 2) ddNTP:...
find the errors
Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at specific locations at the target sequence. The result of digesting a particular genome with a particular restriction enzyme is a collection of restriction fragments of defined length and composition. These can be used to generate restriction maps or create pieces with sticky ends. These sticky ends can be used to attach to other fragments that have sticky ends caused by cutting with a different...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
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