Homework Answers
Answer:- Option C ( met- arg+)
Explanation:- since strain B grows only in medium containing argenine whereas in methionine medium it does not show growth. Whereas when both are present in minimal medium it shows growth but only argenine is required for growth whereas methionine is required for strain A not for strain B.
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The following picture shows growth of five strains of bacera (A,B, C, D, and E) on...
The table below presents the ability of five strains of Neurospora crassa that are deficient in...
The table below presents the ability of five strains of Neurospora crassa that are deficient in arginine biosynthesis to grow on media supplemented with molecules that are biochemical intermediates in the arginine synthesis pathway (“+” = growth, “-“ = no growth). a) Based on these data, diagram both the biochemical intermediates and genes in the arginine synthesis pathway. Mutant strain minimal media +arginine +citrulline +ornithine argA - + - - argB - + ...
Also, How far apart are the bob and billy genes that control growth in the absence...
Also, How far apart
are the bob and billy genes that control growth in the absence of
BOB and BILLY nutrients? Explain your reasoning.
.(4 points) Strain A of bacteria is an Hfr strain that can grow on minimal media without added nutrients, but is sensitive to killing by Ampicillin (genotype Hfr AmpSbobt billyt joet). Strain B of bacteria is an F- strain that is resistant to Ampicillin, and needs the nutrients BOB, BILLY, and JOE added to minimal media...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
A. A B. B C. A or B D. A and B . It depends if...
A. A B. B C. A or B D. A and B . It depends if the organism is prokaryotic or eukaryotic. 7. You have performed the following mating experiment using Hfr and F- strains of Escherichia coli: Hfr (thr+ leu- strs) x F- (thr- leu- str). Which of the following selective media would you use to grow only recombinant colonies? A) Complete media B) Minimal Media C) Minimal Media + streptomycin D) Minimal Media + leucine E) Minimal Media...
Four auxotrophic mutant strains of E. coli (1, 2, 3, 4) are each blocked at a...
Four auxotrophic mutant strains of E. coli (1, 2, 3, 4) are each blocked at a different step of the same metabolic pathway. This pathway involves compounds L, M, N, O, and P. Each of these compounds is tested for its ability to support growth of mutant strains, with the results given below. (+= growth, __ = no growth) Compound Used as a Supplement L M N O P Mutant 1 + __ + + __ Mutant 2 __ __...
The table above summarizes the results of a replica-plating experiment where a mixture of 347 E....
The table above summarizes the results of a replica-plating
experiment where a mixture of 347 E. coli strains of different
genotypes were tested for their ability
to growth on a variety of media. Based on the data presented in the
table above: A. how many colonies are met(+) arg(-)? B. how many
colonies are amp(R) met(-) arg(+)? C. how many colonies are amp(R)
met(-) arg(-)? D. how many colonies are amp(S) met(+) arg(+)? can
you give a detail explanation? I...
Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are...
Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are mated to an F- strain with the genotype StrR, lacZ-, a, b, c, d. Samples of conjugating strains are collected and disrupted every minute for one hour using a blender. Cells are plated under conditions that allow the lacZ+, A, B, C, and D alleles to be identified. The time at which each dominant allele is transferred is shown in the table. lacZ A...
Also, How far apart
are the bob and billy genes that control growth in the absence of
BOB and BILLY nutrients? Explain your reasoning.
.(4 points) Strain A of bacteria is an Hfr strain that can grow on minimal media without added nutrients, but is sensitive to killing by Ampicillin (genotype Hfr AmpSbobt billyt joet). Strain B of bacteria is an F- strain that is resistant to Ampicillin, and needs the nutrients BOB, BILLY, and JOE added to minimal media...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
A. A B. B C. A or B D. A and B . It depends if the organism is prokaryotic or eukaryotic. 7. You have performed the following mating experiment using Hfr and F- strains of Escherichia coli: Hfr (thr+ leu- strs) x F- (thr- leu- str). Which of the following selective media would you use to grow only recombinant colonies? A) Complete media B) Minimal Media C) Minimal Media + streptomycin D) Minimal Media + leucine E) Minimal Media...
The table above summarizes the results of a replica-plating
experiment where a mixture of 347 E. coli strains of different
genotypes were tested for their ability
to growth on a variety of media. Based on the data presented in the
table above: A. how many colonies are met(+) arg(-)? B. how many
colonies are amp(R) met(-) arg(+)? C. how many colonies are amp(R)
met(-) arg(-)? D. how many colonies are amp(S) met(+) arg(+)? can
you give a detail explanation? I...
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