Question

Analyze the following sequence of DNA by answering the related questions: aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tuttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt togaggccgc gattaaattc caacatggat getgatttat atgggtataa atgggctcgc aggtcggaaa tatttatggg cattattatg cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcagtcgac gcggatagct agcaaaggag aagaactctt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccetta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttca cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac cettgttaat agaategagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa atgagagctc caattcgccc gaattcgaat gccagatctg tttagettgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactg a. Identify this sequence? And paste a screen capture for the evidence. b. Predict the translation of the correct ORF and its expected MW in kDa c. Find the restriction map for the DNA sequence using hexacutters restriction enzymes.

Answer 1

Answer:

As the sequence of DNA has been provided in a picture format, it is not possible to copy the sequence in database for its identification and further analysis.

Therefore, I can recommend you few steps which can be followed to obtain the answers of your question.

1, To identify the sequence, go to the NCBI Blast (there is blatn for neucleotide blast and blastp for protein blast).

Note: BLAST: Basic Local Alignment Search Tool.

You need to go to neucleotide Blast of NCBI and paste your DNA sequence in the query box. Now you can click on blast with ticking the option of highly smilar sequence. In the next page you will get several matches to your query with different values for identity percentage. Now choose the option with highest or 100% identity value.

You may capture this screen for evidence as well.

2. Translation is the process of protein synthesis from mRNA.ORF is known as Open Reading Frame (ORF is the neucleotide sequence that is translated to a protein and it begins with ATG as start codon and ends with a stop codon: either UGG, UGA or UAA).

From the given nucleotide sequence, you can easily find the correct ORF using online bioinformatic tools.

First you copy the whole sequence and go to Expasy translate tool page in your browser.

In this tool, paste your sequence and click on translate tab to obtain various reading frames with your neucleotides getting translated into amino acids from N to C terminal.

Every sequence can be translated into three reading frames as every amino acid is coded by three consecutive neucleotides (a single codon). So, in every sequence, the firt reading frame begins with first neucleotide, second with second neucleotide and third with third neucleotide, but fourth neucleotide would form the part of codon which would be included in first frame.

Hence, in this regard, chose the best fit reading frame as ORF, the begining amino acid should be Methionine (Met/M).

3. For obaining the restriction map of your given sequence, paste the neucletides in the box of "NEBcutter" and click on Submit to get the restriction sites as well as the restriction map. A table of Restriction enzyme cutters and the sites for cutting will be provided which can be referred for tracing the hexacutter sites.

Thank you.

If any problem arises while solving, let me know by commenting below.

Give a thumbs up if you are able to understand this.