Homework Answers
1. Recombination refers to the process of putting DNA molecule into a living cell with the help of enzymes.
2. DNA is transferred from a gel to a nylon and hybridised to probe.
3. The complementary sequence will be 3'- GGTACC -5'. And yes this sequence is palindrome since it reads the some in the direction 5' to 3' in both the strands.
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The process of putting a recombinant DNA molecule into a cell is called... Select one: Recombination...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
Write true or false ______ 1. The DNA sequence of one human being is on average...
Write true or false ______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being. ______ 2. As of 2009, all living human beings have had their entire genome sequenced. ______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C. ______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s. ______ 5. A restriction enzyme is useful because it is a generic enzyme...
draw a southern blot in which genomic DNa frow WW rabbits, Ww rabbits pink, and ww...
draw a southern blot in which genomic DNa frow WW rabbits, Ww
rabbits pink, and ww rabbits white has been digested with EcoRI and
hybridized to probe A
3. You have discovered a coat color gene ("W" gene) in rabbits that has two alleles, W and w. Rabbits that are homozygous for W (WW) are red. white. Heterozygous rabbits (Ww) are pink. Rabbits that are homozygous for w (ww) are You have also identified a DNA sequence of about 100...
The partial sequence of one strand of a double stranded DNA molecule is: 5’ ---GACGAAGTGCTGCAGAAAGTCCGCGTTATAGGCATGAATTCCTGAGG--- 3’...
The partial sequence of one strand of a double stranded DNA molecule is: 5’ ---GACGAAGTGCTGCAGAAAGTCCGCGTTATAGGCATGAATTCCTGAGG--- 3’ Write the sequence of both strands of the DNA fragment when this DNA is cleaved with both EcoRI and PstI. The top of your duplex DNA fragment should be derived from the standard sequence given above.
QUESTIONS During replication the protein that keeps the two stands of DNA from popping back together...
QUESTIONS During replication the protein that keeps the two stands of DNA from popping back together is called Gene Semiconservative SSB Single Stranded Binding Protein ODNA polymerase QUESTION 2 Tachnow molecule of DNA is a double he which is made up of O two new stands of DNA one new strand and one old strand of DNA two old stands of DNA o the new strands of DNA QUESTIONS QUESTION 19 The process of synthesizing RNA from a specific sequence...
In RNA interference studies, the double-stranded RNA Select one: a. disrupts the target DNA sequence b....
In RNA interference studies, the double-stranded RNA Select one: a. disrupts the target DNA sequence b. results in the destruction of the target mRNA c. destroys the target protein d. all of the above Hint: it's not D
Only answers please don’t show work 33) Viral DNA incorporated into host cell DNA is known...
Only answers please don’t show work
33) Viral DNA incorporated into host cell DNA is known as a/an A) phage. B) envelope C) capsid. 33) 34) How is transformation in bacteria most A) the creation of a strand of RNA from DNA molecule B) the type of semiconservative by DNA C) the infection of cells by a phage DNA molecule D) the e of a strand of DNA from an RNA molecule E) assimilation of external DNA into a cell...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in process called DNA curs in the nucleus of s acest FS Parent strand Parent strand Newly replicated DNA Newly replicated DNA- SA0 Daughter DNA molecule Daughter DNA molecule Figure 8.2: Overview of DNA replication and illustration of complementary base pairing. DNA must replicate before cell division so that each new daughter cell receives an exact copy of the parent DNA. 1. Replication begins when...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? What is the amino acid sequence of the proteins? Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not? If the T underlined in the above sequence was to go...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
1. An enzyme used to covalently join DNA segments to form recombinant DNA molecules is called a A. Restriction endonuclease B. Reverse transcriptase C. DNA polymerase D. Helicase E. Ligase 7. The procedure for introducing changes into specific genes is called A. An enhancer trap B. Imprinting C. RT-PCR D. DNA looping E. Gene targeting 2. Plasmids used for in vitro cloning of foreign DNA fragments are called A. Donors B. DNA chips C. Clones D. Vectors E. Conjugants 8....
draw a southern blot in which genomic DNa frow WW rabbits, Ww
rabbits pink, and ww rabbits white has been digested with EcoRI and
hybridized to probe A
3. You have discovered a coat color gene ("W" gene) in rabbits that has two alleles, W and w. Rabbits that are homozygous for W (WW) are red. white. Heterozygous rabbits (Ww) are pink. Rabbits that are homozygous for w (ww) are You have also identified a DNA sequence of about 100...
QUESTIONS During replication the protein that keeps the two stands of DNA from popping back together is called Gene Semiconservative SSB Single Stranded Binding Protein ODNA polymerase QUESTION 2 Tachnow molecule of DNA is a double he which is made up of O two new stands of DNA one new strand and one old strand of DNA two old stands of DNA o the new strands of DNA QUESTIONS QUESTION 19 The process of synthesizing RNA from a specific sequence...
Only answers please don’t show work
33) Viral DNA incorporated into host cell DNA is known as a/an A) phage. B) envelope C) capsid. 33) 34) How is transformation in bacteria most A) the creation of a strand of RNA from DNA molecule B) the type of semiconservative by DNA C) the infection of cells by a phage DNA molecule D) the e of a strand of DNA from an RNA molecule E) assimilation of external DNA into a cell...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in process called DNA curs in the nucleus of s acest FS Parent strand Parent strand Newly replicated DNA Newly replicated DNA- SA0 Daughter DNA molecule Daughter DNA molecule Figure 8.2: Overview of DNA replication and illustration of complementary base pairing. DNA must replicate before cell division so that each new daughter cell receives an exact copy of the parent DNA. 1. Replication begins when...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? What is the amino acid sequence of the proteins? Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not? If the T underlined in the above sequence was to go...
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