Hypothesize about why restriction enzyme digestions are performed at 37 degrees Celcius.
Homework Answers
This is because 37 degree celsius is an optimum temperature for the working of restriction enzymes.
These enzymes are isolated from bacteria which live under normal climatic conditions. Under these conditions, the enzymes present in this bacteria will work to their maximum potential. Also the temperature they live in around 30 to 35 degree celsius.
Therefore we perform restriction digestion at 37 degree Celsius so that it can work efficiently at this optimum temperature.
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Hypothesize about why restriction enzyme digestions are performed
at 37 degrees Celcius.
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reference. also, hypothesize what will occur in the presence of an
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Day 1, You have isolated DNA from 30 individuals and performed
restriction enzyme digestion. You have loaded the product and run
the gel electrophoresis following the instruction properly. You
expected to see DNA bands after the procedure is completed, like
figure A, but after the completion of the run your gel looks like
figure B. You did not see any DNA band. You were so upset, but your
lab partner fixed the problem and your gel looked like figure A....
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College students performed the following set of restriction digests on their plasmid pBLA 230. The following below represents the enzyme, the number of bands produced, and the size of the DNA after going through gel electrophoresis. Draw the restriction map of the double digest.
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
Day 1, You have isolated DNA from 30 individuals and performed
restriction enzyme digestion. You have loaded the product and run
the gel electrophoresis following the instruction properly. You
expected to see DNA bands after the procedure is completed, like
figure A, but after the completion of the run your gel looks like
figure B. You did not see any DNA band. You were so upset, but your
lab partner fixed the problem and your gel looked like figure A....
Unanswered Question 6 0/1 pts A restriction digest is performed on a plasmid. The table below shows the sizes of the resulting DNA fragments. 3 enzyme(s) present in the DNA size fragments digest resulting Pstl 10,000 base pairs (bp) Sspl 7,000 bp, 3,000 bp double digest, Estland 4,000 bp, 3,000 bp Sspl Explain the double digest results, what happened to create pieces of these sizes? Your Answer: 4/4 pts Question 7 List the 5 components needed to perform a PCR...
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