Restriction enzymes are tools that aid researchers in analyzing DNA samples.While everyone's DNA is truly unique, there are certain areas of your DNA that you share with every other person on the planet. Since scientists know the order or sequence of those bases, they have developed chemicals known as restriction enzymes that seek out those spots. So when a scientist is analyzing someone's DNA, they add restriction enzymes that find those spots that everyone has and the enzyme cuts the DNA strand into two parts.This results in different numbers of "chunks" of varying lengths/sizes.
Restriction enzymes attach to DNA and are activated by restriction sequences in the DNA. Once activated, the restriction enzymes hydrolyze and destroy the bonds between nucleotides. The restriction sequences along the DNA are inherited, thus, people who are related have similar restriction sequences along their DNA. Cutting DNA samples by the same restriction enzymes and analyzing the resulting DNA fragments by DNA fingerprinting indicates which DNA samples have similar restriction sequences.
Examples of restriction enzyme: EcoRI. It cleaves at the GAATTC recognition sequence.
why is this specific type of restriction enzyme useful for dna fongerprinting anaylisis?
14. A restriction enzyme (for example BamH1): (2 points) a. Is a specific sequence of DNA b. Can be used to ligate DNA ends C. Recognizes palindromic DNA sequences d. Cuts random sequences of DNA
Why do restriction enzymes need to be kept on ice? What order should the DNA, enzyme, water and buffer be added to the microcentrifuge tube for a restriction digest? If lambda DNA is linear, how many times would the enzyme have to cut the DNA to generate five DNA fragments? Would a shorter DNA fragment move faster or slower through the agarose gel than a longer fragment? Why?
find the errors
Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at specific locations at the target sequence. The result of digesting a particular genome with a particular restriction enzyme is a collection of restriction fragments of defined length and composition. These can be used to generate restriction maps or create pieces with sticky ends. These sticky ends can be used to attach to other fragments that have sticky ends caused by cutting with a different...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
Of the following steps to make recombinant DNA, which step occurs immediately after a restriction enzyme recognizes specific nucleotide sequences?
1) If a restriction enzyme cuts a circular DNA into five fragments, how many restriction sites are there in the DNA? 2) How many molecules of DNA will be present after 6 cycles of PCR, if you started with one double-stranded DNA molecule? CELL BIOLOGY QUESTIONS!! SHOW WORK PLEASE
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site of 5’GAATTC 3” and the cutting point between G and A. a. How many fragments you will get b. Specify the size (Number of bases) and the sequence of each fragment, pay attention to DNA direction (5’-3’) 5” ACATTGTCCGGGAATTC CGGGCTAGGCAT T GAATTGGAACA GAATTC GGGCCCGATCCGTA 3
True or false: A piece of DNA cut with a restriction enzyme such as Bam H1 is unlikely to be successfully ligated into a plasmid that has been cut with a different enzyme such as EcoR1.
Use a web search to determine where (in terms of the DNA sequence) the restriction enzyme BamH1 cuts the DNA.
If restriction is cleavage of incoming DNA in a bacterium, what is modification. Is it true that Type II restriction endonucleases catalyze DNA hydrolysis within or near the enzyme recognition sequence and always at the same position?