Describe the step by step process of the enzyme DNA topoisomerase II subunit A
Homework Answers
Topoisomerase 2 uses ATP to cut
both the strands of DNA molecule. Example: DNA gyrase. It decreases
the linking number of DNA by 2.
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Describe the step by step process of the enzyme DNA topoisomerase
II subunit A
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the...
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go...
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means. If you speak about something you have...
The topoisomerase DNA gyrase will introduce negative supercoils into DNA. What effect will this enzyme have...
The topoisomerase DNA gyrase will introduce negative supercoils into DNA. What effect will this enzyme have on relaxed, covalently closed DNA? A The DNA will become more susceptible to cutting by an exonuclease like DNAase I (B) The DNA will become overwound C) The treated DNA will migrate farther in an agarose gel than the relaxed DNA. D) Nucleotides will be removed from the circular DNA. E) The relaxed DNA will migrate farther in an agarose gel than the treated...
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments,...
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the function of Helicase, Primase, topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase
1. Describe the mechanism of topoisomerase type I and II. Be able to describe similarities and ke...
1. Describe the mechanism of topoisomerase type I and II. Be able to describe similarities and key differences in their mechanisms. 2. Describe in molecular detail why there is more binding informatin in the major groove vs minor groove.
Part A The sigma (?) subunit binds to the core RNA polymerase enzyme. The function of...
Part
A
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to the promoter of a gene so that transcription can be initiated.
The closeup shows the secondary structure of the sigma (?)
subunit, which consists of four domains. Identify the domains
labeled 1-3.
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to...
topoisomerase drugs have been developed (and still developing) to treat different cancers in humans. If you...
topoisomerase drugs have been developed (and still developing) to treat different cancers in humans. If you were to develop a drug to target topoisomerase II, which step in its process would be most effective to affect cellular function (assuming it is irreversibly bound)? Why would this be the most effective as a drug?
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
The function of the beta subunit is to prevent DNA polymerase III from dissociating from the...
The function of the beta subunit is to prevent DNA polymerase III from dissociating from the template DNA strand while still allowing the polymerase to slide along DNA as replication progresses. Given this function, the alpha helices in the beta subunit are The function of the beta subunit is to prevent DNA polymerase III from dissociating from the template DNA strand while still allowing the polymerase to slide along DNA as replication progresses. Given this function, the alpha helices in...
The topoisomerase DNA gyrase will introduce negative supercoils into DNA. What effect will this enzyme have on relaxed, covalently closed DNA? A The DNA will become more susceptible to cutting by an exonuclease like DNAase I (B) The DNA will become overwound C) The treated DNA will migrate farther in an agarose gel than the relaxed DNA. D) Nucleotides will be removed from the circular DNA. E) The relaxed DNA will migrate farther in an agarose gel than the treated...
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the function of Helicase, Primase, topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase
Part
A
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to the promoter of a gene so that transcription can be initiated.
The closeup shows the secondary structure of the sigma (?)
subunit, which consists of four domains. Identify the domains
labeled 1-3.
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
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