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In order for a human genomic DNA library to fully represent the entire genomic information content...

In order for a human genomic DNA library to fully represent the entire genomic information content of humans, what type of methods must be employed to fractionate the human genomic DNA for making the library?

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A gene library is a large collection of cloned DNA sequences from a single genome. A genomic library which contain at least one copy of every sequence in an organism’s genome, are used to investigate the structure of a given chromosome, or to clone specific genes. Genomic DNA libraries contain large fragments of DNA in either bacteriophages or bacterial or P1-derived artificial chromosomes (BACs and. PACs). In order for a human genomic DNA library to fully represent the entire genomic information content of humans, methods like recombinant DNA technology, cloning, DNA sequencing methods etc must be employed to fractionate the human genomic DNA for making the library. Various hosts like plasmids, bacteriophage lambdas, cosmids, YACs and many more are used to construct such genomic libraries.

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