High throughout DNA sequencing by synthesis requires the use of ________ deoxynucleotides that can be visualized...
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Homework Answers
High throughout DNA sequencing by synthesis is also known as Next-generation sequencing.
It involves following steps.
1. DNA cleavage to produce small fragments.
2. Primer extension in the presence of labelled and blocked nucleotides
3. Separation by electrophoresis and read out of labels.
So, it uses ddNTPs.
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High throughout DNA sequencing by synthesis requires the use of
________ deoxynucleotides that can be visualized...
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known...
Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? Why are next-generation DNA sequencing technologies known as sequencing-by-synthesis? The complete DNA strands are synthesized then sequenced. Numerous synthesized fragments of DNA are sequenced to determine which nucleotides were incorporated. The sequencing occurs during S phase of the cell cycle. RNA molecules are synthesized off of the DNA templates and the incorporated nucleotides are then determined. Incorporated nucleotides are determined while they are being added to a growing DNA strand.
Dideoxy nucleotides that are used in DNA sequencing experiments terminate DNA synthesis because they: cannot be...
Dideoxy nucleotides that are used in DNA sequencing experiments terminate DNA synthesis because they: cannot be recognized by DNA polymerases and therefore are not incorporated during synthesis. have no 3′ -OH group and so no additional nucleotides can be added after these nucleotides are incorporated. have no phosphate groups. are only recognized by reverse transcriptases. have methyl groups attached to the 5′ position of the sugar and cannot be incorporated during synthesis.
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
which if these molecules can be employed as a chain terminator in DNA sequencing through thr...
which if these molecules can be employed as a chain terminator
in DNA sequencing through thr Sanger method?
Which of these molecules can be employed as a chain terminator in DNA sequencing through the Sanger method? 0 - 0- - 0-- - 0- 0-p 0 P - O- P- O- i - iii 0--- 0--- 0- - 0- - 0- 0 0-
The observation that in any DNA sample, A T and G C A. DNase sequencing An...
The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
Which of the following statements about eukaryotic DNA replication is false? A) DNA polymerase can add...
Which of the following statements about eukaryotic DNA replication is false? A) DNA polymerase can add new nucleotides to either free 3'- or 5'-end B) RNA primers must be removed and rejected by the deoxynucleotides C) RNA primers must be laid down during DNA replication for synthesis of new DNA that starts at the 3'-OH of the primer ends D) Elongation of the DNA strand during replication requires primers with free 3'OH end E) The very end of the chromosome...
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer): Select one or more: a. Both technologies use terminators b. Both methods require PCR amplification c. Both methods require a ligation step d. Both methods require a restriction endonuclease e. Both methods require primers f. Both methods were developed by scientists while under the influence of strong hallucinogenic drugs...
36. Which of the following is true for the ribosomes (rRNA) found in Prokaryotic cells? They are: a) made of DNA and protejn, b) the active site in carbohydrate synthesis, c) subunits that use m...
36. Which of the following is true for the ribosomes (rRNA) found in Prokaryotic cells? They are: a) made of DNA and protejn, b) the active site in carbohydrate synthesis, c) subunits that use mRNA as the directions for primary structure of protein attached to the endoplasmic reticulum, e) used to synthesize DNA by dehydration synthesis. answer the question and give a why the circled answer wasn't correct
36. Which of the following is true for the ribosomes (rRNA) found...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are lacking a OH at the...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
which if these molecules can be employed as a chain terminator
in DNA sequencing through thr Sanger method?
Which of these molecules can be employed as a chain terminator in DNA sequencing through the Sanger method? 0 - 0- - 0-- - 0- 0-p 0 P - O- P- O- i - iii 0--- 0--- 0- - 0- - 0- 0 0-
The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
36. Which of the following is true for the ribosomes (rRNA) found in Prokaryotic cells? They are: a) made of DNA and protejn, b) the active site in carbohydrate synthesis, c) subunits that use mRNA as the directions for primary structure of protein attached to the endoplasmic reticulum, e) used to synthesize DNA by dehydration synthesis. answer the question and give a why the circled answer wasn't correct
36. Which of the following is true for the ribosomes (rRNA) found...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
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