Homework Answers
b.
DdNTP refers to Dideoxynucleotides triphosphates which are used in Sanger dideoxy method to produce different lengths of DNA strands for DNA sequencing. When present in small amounts in sequencing reactions, dideoxyribonucleoside triphosphates (ddNTPs) terminate the sequencing reaction at different positions in the growing DNA strands. ddNTPs stop a sequencing reaction because they cause DNA polymerase to fall off the template strand. The deoxyribonucleotides do not have a 3' hydroxyl group, hence no further chain elongation can occur once this dideoxynucleotide is on the chain. This can lead to the termination of the DNA sequence.
Rest of the choices contains OH group on the pentose sugar.
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which if these molecules can be employed as a chain terminator
in DNA sequencing through thr...
When sequencing DNA by chain termination, the terminator nucleotide A) is missing a 2' OH B)is...
When sequencing DNA by chain termination, the terminator nucleotide A) is missing a 2' OH B)is missing a 5' phosphate C)is missing both 2' and 3' OH
32. Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers ...
32. Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are present or (b) an excess of primers is present.
32. Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are present or (b) an excess of primers is present.
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a single nucleotide a. Magnesium b. Heat c. ddNTPs d. restriction enzymes e. none of the above
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Selec...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Select all that apply.)12.17 a. Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. b. Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper. c. A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. d. Duplex nucleic acid molecules can be separated by size by means of electrophoresis. e. New nucleotides are added only to the...
O The Sanger method of DNA sequencing requires which of the following to be in the...
O The Sanger method of DNA sequencing requires which of the following to be in the reaction tube? Choose one: O A. template DNA O B. deoxyribonucleotides O C. dideoxyribonucleotides O D. A, B, and C are correct. O E. Only A and B are correct. O F. Only A and Care correct. O O O
In automated DNA sequencing reaction by the Sanger method, which of the following is NOT a...
In automated DNA sequencing reaction by the Sanger method, which of the following is NOT a component of the reaction? A) fluorescently-labeled deoxynucleotides B) DNA polymerase C) a DNA primer D) the DNA to be sequenced E) none of the above (i.e. all the above are required in the reaction)
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer): Select one or more: a. Both technologies use terminators b. Both methods require PCR amplification c. Both methods require a ligation step d. Both methods require a restriction endonuclease e. Both methods require primers f. Both methods were developed by scientists while under the influence of strong hallucinogenic drugs...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
32. Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are present or (b) an excess of primers is present.
32. Describe the outcome of a chain-terminator sequencing procedure in which (a) too few primers are present or (b) an excess of primers is present.
O The Sanger method of DNA sequencing requires which of the following to be in the reaction tube? Choose one: O A. template DNA O B. deoxyribonucleotides O C. dideoxyribonucleotides O D. A, B, and C are correct. O E. Only A and B are correct. O F. Only A and Care correct. O O O
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
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