When sequencing DNA by chain termination, the terminator nucleotide A) is missing a 2' OH B)is...
When sequencing DNA by chain termination, the terminator nucleotide
A) is missing a 2' OH
B)is missing a 5' phosphate
C)is missing both 2' and 3' OH
Homework Answers
I believe the correct answer to be:
Option C) Is missing both 2' and 3' OH.
And since 3' OH is missing, the polymerase is no longer able to add any nucleotide to the growing chain hence terminating the reaction.
feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.
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When sequencing DNA by chain termination, the terminator
nucleotide
A) is missing a 2' OH
B)is...
which if these molecules can be employed as a chain terminator in DNA sequencing through thr...
which if these molecules can be employed as a chain terminator
in DNA sequencing through thr Sanger method?
Which of these molecules can be employed as a chain terminator in DNA sequencing through the Sanger method? 0 - 0- - 0-- - 0- 0-p 0 P - O- P- O- i - iii 0--- 0--- 0- - 0- - 0- 0 0-
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next...
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next generation sequencing. (5 points)
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing...
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing gel? A C GT a.5' - TTGGCCAAA - 3 6.5' - AAACCGGTT-3' OC. 5'- ATGGACTCA - 3 d. 5'-ACTCAGGTA - 3' e. 5' - TGAGTCCAT - 3'
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
In attempting to determine the composition of a nucleotide sequence, the chain terminating method employs the...
In attempting to determine the
composition of a nucleotide sequence, the chain terminating method
employs the following type of terminator. Why does it work?
It is missing the 2' OH.
The linker is too big to allow further transcription.
It is missing the 5' OH.
It is missing the 3' OH.
It has a triphosphate instead of a monophosphate.
NH Linker HO-P0-P0-P-01 | H OH OH NH9 Dye Nucleotide = Linker between nucleotide and dye
please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a single nucleotide a. Magnesium b. Heat c. ddNTPs d. restriction enzymes e. none of the above
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel a. bottom to top; 5′ to 3′; shorter; early b. top to bottom; 5′ to 3′; longer; early c. bottom to top; 5′ to 3′; longer; later d. top to bottom; 5′ to 3′; shorter; early e. bottom to top; 3′ to 5′; shorter; early 8....
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Selec...
Which of the following make(s) sequencing by the Sanger chain-termination method possible? (Select all that apply.)12.17 a. Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. b. Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper. c. A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. d. Duplex nucleic acid molecules can be separated by size by means of electrophoresis. e. New nucleotides are added only to the...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
which if these molecules can be employed as a chain terminator
in DNA sequencing through thr Sanger method?
Which of these molecules can be employed as a chain terminator in DNA sequencing through the Sanger method? 0 - 0- - 0-- - 0- 0-p 0 P - O- P- O- i - iii 0--- 0--- 0- - 0- - 0- 0 0-
I Q10A. Differentiate between two types of DNA sequencing methodologies, sequencing by chain termination and next generation sequencing. (5 points)
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing gel? A C GT a.5' - TTGGCCAAA - 3 6.5' - AAACCGGTT-3' OC. 5'- ATGGACTCA - 3 d. 5'-ACTCAGGTA - 3' e. 5' - TGAGTCCAT - 3'
In attempting to determine the
composition of a nucleotide sequence, the chain terminating method
employs the following type of terminator. Why does it work?
It is missing the 2' OH.
The linker is too big to allow further transcription.
It is missing the 5' OH.
It is missing the 3' OH.
It has a triphosphate instead of a monophosphate.
NH Linker HO-P0-P0-P-01 | H OH OH NH9 Dye Nucleotide = Linker between nucleotide and dye
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
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