1.A.Describe the difference between F+ bacterium and Hfr bacterium fertility genes. B.How do each of these...
1.A.Describe the difference between F+ bacterium and Hfr bacterium fertility genes. B.How do each of these contribute to bacterial recombination leading to variation?
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1.A.Describe the difference between F+ bacterium and Hfr
bacterium fertility genes. B.How do each of these...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to F recipient bacteria. The data shown below list the origin of transfer (oriT) for each strain and give the order of genes, with the first gene on the left and the last gene on the right. Hfr Strain Genes Transferred Hfr 1 Hfr 2 oriT met pro leu gal oriT ala lac thr azi oriT azi trp met pro Hfr 3 Use the...
Why do different conjugating strains show different gradients of transfer? Group of answer choices A. Hfr...
Why do different conjugating strains show different gradients of transfer? Group of answer choices A. Hfr plasmids pick up different bacterial genes when they excise from the chromosome. B. There is variation in the rate that each F+ strain transfers DNA. C. The Hfr plasmid can integrate into different locations in the bacterial genome creating different F+ strains that initiate transfer at different locations. D. The F+ plasmid can integrate into different locations in the bacterial genome creating different Hfr...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer ikvqw izrbm vkimb 3 Answer Bank Answer...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
You use à new strain of Hfr E. coli to map several genes by using interrupted...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met...
Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met tyr.is crossed with an F strain with genotype val met tyr. Val is known to enter last, and so val" recombinants are selected on medium that contains no val (only met and tyr). Recombinant colonies are then tested for met and tyr". The table summarizes the results. #Colonies 552 valt met tyrt Genotype valt met+tyrt valt met- tyr+ valt met- tyr. valt met+ tyr-...
1. Differentiate between an F+ cell and an Hfr cell in terms of where the F...
1. Differentiate between an F+ cell and an Hfr cell in terms of where the F factor is located (i.e. chromosome or plasmid) and what type of genetic information (plasmid or chromosome) can be transferred during conjugation. F+ cell: Hfr cell:
1) Choose from the following options to complete the passage below: F-, F+, F', Hfr. A(n)...
1) Choose from the following options to complete the passage
below: F-, F+, F', Hfr.
A(n) ___?___ cell, which has the F plasmid incorporated into the
bacterial genome, can only conjugate with ___?____
cells. If the plasmid recircularizes, carrying some of the
bacterial DNA with it, the cell is now considered to be
___?____.
2) If conjugation occurs between the two cells below, what will
be the genotype of the exconjugant cell?
a) F+ lac+
b) F+ lac-
c) F-...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross is Hfr/a+b+c+ rifs ×F−a−b−c− rifr . (No map order is implied in the listing of the alleles; rifr is resistance to the antibiotic rifampicin.) The a+gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, c+ for nutrient C. The cross is initiated at time = 0, and at various times, the mating mixture is plated on medium. Each plate contains minimal medium (MM) plus rifampicin....
1. 2. 13. Which statement explains why the recombination frequency between two genes is always less...
1.
2.
13.
Which statement explains why the recombination frequency between two genes is always less than 50%? Recombination cannot be more than 50% because chromosomes are only 50 map units in length. The genotype of the F, gametes will always be 50% parental gametes and 50% recombinant gametes. Genes with a recombination frequency near 50% are unlinked and have an equal likelihood of being inherited together or separately. F1 gametes always have 50% of their alleles from each parental...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to F recipient bacteria. The data shown below list the origin of transfer (oriT) for each strain and give the order of genes, with the first gene on the left and the last gene on the right. Hfr Strain Genes Transferred Hfr 1 Hfr 2 oriT met pro leu gal oriT ala lac thr azi oriT azi trp met pro Hfr 3 Use the...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer ikvqw izrbm vkimb 3 Answer Bank Answer...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met tyr.is crossed with an F strain with genotype val met tyr. Val is known to enter last, and so val" recombinants are selected on medium that contains no val (only met and tyr). Recombinant colonies are then tested for met and tyr". The table summarizes the results. #Colonies 552 valt met tyrt Genotype valt met+tyrt valt met- tyr+ valt met- tyr. valt met+ tyr-...
1. Differentiate between an F+ cell and an Hfr cell in terms of where the F factor is located (i.e. chromosome or plasmid) and what type of genetic information (plasmid or chromosome) can be transferred during conjugation. F+ cell: Hfr cell:
1) Choose from the following options to complete the passage
below: F-, F+, F', Hfr.
A(n) ___?___ cell, which has the F plasmid incorporated into the
bacterial genome, can only conjugate with ___?____
cells. If the plasmid recircularizes, carrying some of the
bacterial DNA with it, the cell is now considered to be
___?____.
2) If conjugation occurs between the two cells below, what will
be the genotype of the exconjugant cell?
a) F+ lac+
b) F+ lac-
c) F-...
1.
2.
13.
Which statement explains why the recombination frequency between two genes is always less than 50%? Recombination cannot be more than 50% because chromosomes are only 50 map units in length. The genotype of the F, gametes will always be 50% parental gametes and 50% recombinant gametes. Genes with a recombination frequency near 50% are unlinked and have an equal likelihood of being inherited together or separately. F1 gametes always have 50% of their alleles from each parental...
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