Answer 1: When staining a thick smear made from uneven cell suspension then it will be difficult to see any bacteria bacause due to overlapping between cell staining is not proper therefore, proper reading of gram bacteria is difficult.
Answer 2: On using old gram crystal violet prepration containing crystalized dye then dye would not properly penetrate the membrane.
Answer 3: On using aqueous crystal violet in place of gram crystal violet then dyes would not deposited,it just washed off.
What effect do you suspect the following will have on your judgment of the Gram reaction...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
- Which of the following pairs is mismatched? d. iodine mordant e. none of the above a. alcohol-decolorizer b. crystal violet-basic dye c. safranin-acid dye 2, Suppose you Gram stained a sample from one colony and observed a field of red and blue cocci. Adjacent cells were not always the same color. You could conclude that you have d. old cells e. made a mistake a. a mixed culture b. young cells c. dividing cells 3. Which of the following...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what was done A summary of the most important results A sentence stating the major conclusion EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
100. Flare is: (a) the widest frequency of light that can enter an objective (b) the bending of light as it passes through different substances (c) wavelength/2NA (d) when the beam of light "spreads" wider than the objective's "hole" (e) wavelengths of light too short to enter the objective 101. Coccobacillus shaped bacteria are considered to be_?_ (a) cocci (b) bacilli (c) diplobacilli (d) diplococci (e)answers a and b only 102. This microscope is used for cheap and quick "'diagnosis"...