Homework Answers
Request Answer!
We need at least 10 more requests to produce the answer.
0 / 10 have requested this problem solution
The more requests, the faster the answer.
Add Answer to:
would you expect transformed cells?
Plate # Label Growth Transformed Cells Yes 2 yes +PGLO LBlamp...
.1)a) Why was it important that the –pGLO bacteria were found growing on the LB plate?...
.1)a) Why was it important that the –pGLO bacteria were found growing on the LB plate? Why would their absence create problems in interpreting our results? b) What two conclusions (one per plate) should you draw from the observation that –pGLO bacteria did not grow on the LB/amp plate, but that some +pGLO bacteria did? c). Why did we observe bacteria colonies that glowed under UV light when we inoculated +pGLO bacteria on an LB/amp/ara plate, but not when we...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
9. On which of the following plate, both transformed and non-transformed cells grow? A. LBAB.LBA+amp C....
9. On which of the following plate, both transformed and non-transformed cells grow? A. LBAB.LBA+amp C. LBA+amp+X-Gal 10. On which of the following plate, only transformed cells can grow? A. LBAB.LBA+amp C. LBA+amp+X-Gal D. both B and C E. none 11. On which of the following plate, only non-transformed cells grow? A. LBAB.LBA+amp C. LBA+amp+X-Gal D. both B and C E. none
1. On which plate(s) would you expect to find bacteria most like the E. coli on...
1. On which plate(s) would you expect to find bacteria most like the E. coli on the source plate? Explain. 2. On which plate(s) would you find only genetically transformed bacterial cells? Why? 3. What is the purpose of the control plates? Explain the difference between the controls and why each one is necessary. 4. Why would one compare the -DNA/+Amp and DNA/+Amp plates?
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of...
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of 0.08 ug ul. You transformed 191 UL of competent E. coli with 100L of PGLO DNA. You added 191 UL of LB broth to the transformation before plating 100 uL of the culture to a plate labeled +POLO, LB, Amp, Ar. After growing the plates overnight, 136 fluorescent colonies (transformants) were counted. What was the average transformation efficiency of your work?
12a. If you plated transformed cells on LB, what would the growth look like? Why? b....
12a. If you plated transformed cells on LB, what would the growth look like? Why? b. How else could growth occur on plates with both antibiotics with regard to “successful” transformation? (Hint: How else could you both genes “get into” bacterial cells?) c. What other ways can growth occur on plates with both antibiotics () that do not have anything to do with transformation/molecular cloning?
Question #1. Lab 12: Transformation GFP Results Table 1. Transformation Results Color of Color of Number...
Question #1.
Lab 12: Transformation GFP Results Table 1. Transformation Results Color of Color of Number of Plate colonies under colonies under coloniesc UV light normal light co LB (-pGLO) LB/amp (-pGLO LB/amp +pGLO beige C LB/amp/ara (+pGLO) s beige Avololnose Table 2. Calculating transformation efficiency Number of transformed colonies (# transformants on LB/amp/ara plate) Transformation efficiency Mass of plasmid plated (transformants/ug plasmid) 0.156 750156 480 27 H Questions: Did you transform bacteria? Use your experimental results as evidence to...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
1. On which plate do you expect bacteria to glow when viewed under UV light? Which...
1. On which plate do you expect bacteria to glow when viewed under UV light? Which will grow more colonies than others? Fill in applicable options: all glow / some glow, some don't / none glow / more colonies / less colonies Plate conditions LB with Ampicillin plate LB no Ampicillin plate none glow more colonies Both gfp transformed and untransformed bacteria in normal light Both gfp transformed and untransformed bacteria in UV light
9. On which of the following plate, both transformed and non-transformed cells grow? A. LBAB.LBA+amp C. LBA+amp+X-Gal 10. On which of the following plate, only transformed cells can grow? A. LBAB.LBA+amp C. LBA+amp+X-Gal D. both B and C E. none 11. On which of the following plate, only non-transformed cells grow? A. LBAB.LBA+amp C. LBA+amp+X-Gal D. both B and C E. none
1. On which plate(s) would you expect to find bacteria most like the E. coli on the source plate? Explain. 2. On which plate(s) would you find only genetically transformed bacterial cells? Why? 3. What is the purpose of the control plates? Explain the difference between the controls and why each one is necessary. 4. Why would one compare the -DNA/+Amp and DNA/+Amp plates?
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of 0.08 ug ul. You transformed 191 UL of competent E. coli with 100L of PGLO DNA. You added 191 UL of LB broth to the transformation before plating 100 uL of the culture to a plate labeled +POLO, LB, Amp, Ar. After growing the plates overnight, 136 fluorescent colonies (transformants) were counted. What was the average transformation efficiency of your work?
Question #1.
Lab 12: Transformation GFP Results Table 1. Transformation Results Color of Color of Number of Plate colonies under colonies under coloniesc UV light normal light co LB (-pGLO) LB/amp (-pGLO LB/amp +pGLO beige C LB/amp/ara (+pGLO) s beige Avololnose Table 2. Calculating transformation efficiency Number of transformed colonies (# transformants on LB/amp/ara plate) Transformation efficiency Mass of plasmid plated (transformants/ug plasmid) 0.156 750156 480 27 H Questions: Did you transform bacteria? Use your experimental results as evidence to...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate...
1. On which plate do you expect bacteria to glow when viewed under UV light? Which will grow more colonies than others? Fill in applicable options: all glow / some glow, some don't / none glow / more colonies / less colonies Plate conditions LB with Ampicillin plate LB no Ampicillin plate none glow more colonies Both gfp transformed and untransformed bacteria in normal light Both gfp transformed and untransformed bacteria in UV light
Most questions answered within 3 hours.
-
4) In a polypeptide, which bond cannot rotate because of its
partial double bond character?
The...
asked 25 minutes ago -
Assume that in the short run L = 1,000 and K = 100. 1. What is...
asked 20 minutes ago -
At a given temperature, 2.06 atm of H2 and 3.7 atm of Br2 are
mixed and...
asked 19 minutes ago -
Sodium reacts with Hydrochloric acid to form sodium chloride and
hydrogen gas. 2Na(s)+ 2 HCl(aq)-> 2...
asked 26 minutes ago -
The following circuits (1 & 2) are combined to form a
series-parallel circuit and resulting circuit...
asked 34 minutes ago -
Feynman's use of path integrals can make some of the transition
from quantum scale to real...
asked 33 minutes ago -
Pb(NO3)2 (aq) + 2 KCl (aq) PbCl2 (s) + 2 KNO3 (aq)
If 54.5mL of 3.82M...
asked 34 minutes ago -
Razeghi (2008) states "In order to succeed at innovation, do not
focus on being creative; rather...
asked 40 minutes ago -
How much heat is required at constant pressure to melt 1 mole of
ice at -25...
asked 43 minutes ago -
Suppose N is a discrete random variable defined by probability
distribution:
n
Pr(N = n)
10...
asked 44 minutes ago -
why are special-interest travelers becoming more important to
tourism service suppliers?
asked 48 minutes ago -
A 50.0-kg child takes a ride on a Ferris wheel that rotates four
times each minute...
asked 1 hour ago