In a wild type Drosophila melanogaster embryo, which cells have a nuclear localization of the Dorsal protein?
During the process drosophila embryogenesis the most important step is dorsal protein localization and it occurs in the cytoplasm of the nuclei of the ventral cells during the 14th division cycle.
In a wild type Drosophila melanogaster embryo, which cells have a nuclear localization of the Dorsal...
For proteins that normally are nuclear, deletion of the nuclear localization signal and injection of the protein into the cytoplasm of cultured cells will result in localization A. that is extracellular. B. in a ring around the outside of the nuclear envelope. C. in the nucleus. D. in the cytoplasm.
The engrailed gene of Drosophila is expressed in 14 stripes in the embryo. This corresponds to a line of cells in each segment. If one makes a fly where all of the engrailed enhancers drive the expression of GFP (green fluorescent protein) you predict: A. The embryo will have evenly green fluorescence over its entirety. B. The embryo will have 7 stripes of green fluorescence. C. The embryo will have 14 stripes of green fluorescence. D. There will be no...
In a cross involving Drosophila melanogaster, an F_2 population included 4400 flies with normal wings and 1524 flies with short wings. Calculate: A. X^2 (chi square) B. Degrees of freedom C. The probability (P-Value) D. Does your data support the null hypothesis or reject and why? In a dihybrid cross involving Drosophila melanogaster, an apterous (wild type eyes, no wings) female was crossed with a sepia (brown dark eyes, wild type wings) male. An F_2 population included Calculate: A. X^2(chi...
CRISPR-Cas9 technology is used to engineer Drosophila melanogaster in which the Sx1 protein contains an RS domain. Will flies homozygous for this mutation be male or female? Explain.
Finally, bow-legs is hypothesized to be X-linked recessive in Drosophila melanogaster. The P1 virgin females were, once again, homozygous wild type but the males were bow-legged. There were 52 wild type males and 67 wild type females in the F1 generation. The F2 generation contained 30 wild type males, 75 wild type females, 40 bow-legged males and no bow-legged females. Does this data support or reject the hypothesis? Use chi square to prove your position.
What is protein X and what gene could be mutated to cause these results? Wild type Mutant DNA Protein X DNA Protein X Compound Compound Untreated US Protein X is a nuclear hormone receptor. The mutation is in protein X and it no longer binds compound S. Protein X is a nuclear hormone receptor. The mutation is in protein X and increases its affinity for compound Protein X is NFAT. The mutation is in the GPCR that binds compound U...
Drosophila melanogaster normally have four sets of chromosomes. What total number of chromosomes (actually the total number of chromatids is a more accurate way to phrase this) will the four gamete cells have after a single non-disjunction event in MII? Write four numbers separated by commas
4. In Drosophila melanogaster, the phenotype curly wings is due to a mutant allele Cy that is lethal when homozygous. A population is established with an initial frequency of Cy equal to 0.168. Denoting + as the wild- type (i.e. non-mutant) allele at this locus, calculate the expected frequency of Cy in the next generation if the relative fitness of the ++ homozygote to the Cy/+ heterozygote is: a) 1 : 1 b) 1 : 0.5 c) Briefly explain why...
The phenotype of vestigial (short) wings (vg) in Drosophila melanogaster is caused by an autosomal recessive mutant allele that independently assorts with an autosomal recessive mutant allele for hairy (h) body. A parental cross was made between a fly that is homozygous for normal wings with a hairy body and a fly with vestigial wings that is homozygous for normal body hair. The wild-type F1 flies were crossed to each other and produced 1536 offspring. The phenotypes of the F2...
The phenotype of vestigial (short) wings (vg) in Drosophila melanogaster is caused by an autosomal recessive mutant allele that independently assorts with an autosomal recessive mutant allele for hairy (h) body. A parental cross was made between a fly that is homozygous for normal wings with a hairy body and a fly with vestigial wings that is homozygous for normal body hair. The wild-type F1 flies were crossed to each other and produced 1536 offspring. The phenotypes of the F2...