What approximate fraction of the low-power field AREA would you see if you were to change to the high-power objective
4. What approximate fraction of the low-power field AREA would you see if you were to change to the high-power objective, using the microscope from Question 3? Keep it simple! And remember: A = (pi/(r2). Show calculations.
Homework Answers
Field of view (diameter that can be seen at given magnification) is inversely proportional to magnification. Higher the FOV, lower the magnification and vice versa.
(FOV)1 (M)1 = (FOV)2 (M)2
FOV1 = 1600 um
M1 = 10x
FOV2 = ?
M2 = 40x
(1600)(10x) = (FOV)2(40x)
FOV2 = 400 um
At 40x, you will see 400 um of the area.
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What approximate fraction of the low-power field AREA would you see if you were to change to the high-power objective
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3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a. The first lens that provides magnification of the image is the c lor lens. b. The lens that further enlarges the image ten more times is the low power nel lens. 4. Open the iris diaphragm all the way while using the low power objective. What happens to the field of view and the quality of the image? Now close the iris diaphragm all...
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Lab activities and worksheets Read through this section before you get to lab so you are aware of what you will be doing for this lab. Microscope labeling It is important to know the structure of something in order to know how it works and how to use it. Use the following list of parts of a compound microscope to label the diagram. Be sure to understand the functions of each part. Ocular: this is the lens at the top...
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