Question

how do you properly come up with the answers to the highlighted questions?

rebnuary 25,2019IO 9) (10 points) You are trying to determine if the amount of protein the manufacturer says it supplies in baby formula is actually correct. Using the Bradford assay, you have constructed the standard curve shown below. You make a 1:100 dilution of baby formula in phosphate buffer. You then measure the absorbance of 10, 20, or 40 μ1 of the dilution solution. ao)xcio) xo) Sample amount ( 0.20 40 Using the standard curve, determine how much Remember that y mx+b Abs Asbic F 20? Hou? Thens determine the amount of proteln in he baby oemuin mean 4l protein is in your samples BSA Standard Cruve 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 203040 S00 700 ul of BSA

Answer 1

The traditional method for calculating protein concentration of an unknown sample is to use a standard curve that is generated from known protein standards.   A linear regression for the set of standards as they assume the overall relationship between the standards is best described by a linear relationship (R2=0.9791), which is rarely seen. The red line in the Linear Plot figure shows the linear plot and the equation for this line is y=1.0736x + 0.0677. Solving for x for an absorbance will give the protein concentration

a) for 10 ul, the absorbance is 0.2, so the amount of protein concentration

x= (Y-0.0677)/1.0736=( 0.2-0.0677)/1.0736=0.12 ug

for 20 ul the protein amount is 0.29 ug

for 40 ul the protein amount is 0.63 ug

B) since the dilution was 1:100

the total amount of protein in each reaction is

0.12x100=12ug

0.29x100=29ug

0.63x100=63ug.