Question

introduction about effet of parameters ( ph,Tm,incubation,concentration, inhibitors and substrate ) on ACP enzyme ctalyzed reaction

Answer 1

# Factors affecting ACP enzyme catalysed reaction

# pH; Every enzyme has its own optimum pH to perform their function effectively. Some enzymes are active at physilogical pH 7.4 (salivary amylase) and some are active at acidic pH (pepsin). So pH less or higher than physilogical pH would affect the rate of ACP enzyme catalysed reaction.

# Temperature (Tm); All enzymes function at a range of temperature only. This is called as optimum temperature (37 degree celsius). Higher than this temperature causes denaturation of enzyme and lower than this leads to reduced function.

# Incubation; Rate of reaction increases with increasing incubation time with substrate at the beginning but, after a stage the rate falls down.

# Concentration; Rate of reaction increases with increased concentration of enzymes because of the availability of more active sites for substrate.

# Inhibitor; An inhibitor always interfere with the product formation. It competes with the substrate for active site or it changes the conformation of an enzyme by binding to other than the active site.

# Substrate; Rate of reaction increases with increased substrate concentration but after reaching maximum velocity the rate of reaction remains unchanged (will not increase further). This is because the unavailability of active sites at enzyme for substrate.