Craig performed a side-by-side Gram satin of a known Gram positive and known Gram negative organism....
Craig performed a side-by-side Gram satin of a known Gram positive and known Gram negative organism. Unfortunately, he forgot to do iodine step. When he observed his completed slide under oil immersion, what do you think he saw?
For microbiology
Homework Answers
In absence of iodine solution, crystal violet - iodine complex not formed. Then the destaining reagent (acetone) would remove the purple stain from Gram positive and Gram negative cells.
Thus, both Gram positive and Gram negative would appear as like counterstain colour - pink colour. (Counterstain * Safranin and * Basic fuchsin)
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Craig performed a side-by-side Gram satin of a known Gram
positive and known Gram negative organism....
1. a) Contrast the concepts of magnification, refraction, and resolution b) How can one obtain 2,000X...
1. a) Contrast the concepts of magnification, refraction, and resolution b) How can one obtain 2,000X magnification with a 100X objective? 2. Craig performed a side-by-side Gram stain of a known gram-positive and known gram-negative organism. Unfortunately, he forgot to do iodine step. When he observed his completed slide under oil immersion, what do you think he saw? 3. What are the advantages and disadvantages of observing living-specimens slides and heat-fixed-specimen slides of microorganisms?
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells...
0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple- colored cells are observ ed? you forgot the mordant step O you killed the cells during heat fixation you forgot the decolorization step you forgot to add immersion oi
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
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QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Help with 1,3 4,5and 7 please God bless u morphology morphologies Ex. 5 Questions 1. Gram-positive...
Help with 1,3
4,5and
7 please God bless u
morphology morphologies Ex. 5 Questions 1. Gram-positive cultures that are older than 24hrs can stain as Gram negative sometimes. Why does this happen? 2. Why is iodine important in the Gram stain? 3. Suppose you do a Gram stain on a pure culture of Gram-positive cocci. When you view the What should slide, you see some purple spheres and red spheres adjacent to one another. you conclude and why? 으..@ 흐...
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NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
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0/1 pts Incorrect Question 8 You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple- colored cells are observ ed? you forgot the mordant step O you killed the cells during heat fixation you forgot the decolorization step you forgot to add immersion oi
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Help with 1,3
4,5and
7 please God bless u
morphology morphologies Ex. 5 Questions 1. Gram-positive cultures that are older than 24hrs can stain as Gram negative sometimes. Why does this happen? 2. Why is iodine important in the Gram stain? 3. Suppose you do a Gram stain on a pure culture of Gram-positive cocci. When you view the What should slide, you see some purple spheres and red spheres adjacent to one another. you conclude and why? 으..@ 흐...
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NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
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