What is the location of the fluorophore part of GFP in relation to the polypeptide chain of GFP?
Fluorophore is a tripeptide consisting residues serine, tyrosine and glycine at position 65-67 in the sequence.
What is the location of the fluorophore part of GFP in relation to the polypeptide chain...
Provide a rough estimate of the molecular weight of the fluorophore part of GFP as a percentage of the GFP molecular weight: MWfluorophore ≈ __________________% of MWGFP
You want to tag the extracellular part of a single-pass transmembrane receptor with GFP, which requires that you modify the DNA sequence encoding the receptor to also include the DNA sequence encoding GFP. a) Would you tag the receptor so that GFP is fused to that portion of the polypeptide that is translocated into the ER lumen during translation? Or instead, would GFP be fused to that portion of the polypeptide that remains in the cytosol during translation? (2 points)...
The ER signal sequence on a growing polypeptide chain is recognized by a signal-recognition particle (SRP) in the cytosol. What does this interaction accomplish? It cleaves the ER signal sequence from the polypeptide chain. It cleaves the ER signal sequence from the polypeptide chain. It guides the ribosome and its polypeptide to the ER. It releases the polypeptide chain from the ribosome. It speeds the synthesis of the polypeptide chain. Which of the following choices reflects the appropriate order of...
The mitochondria precursor protein is imported as an unfolded polypeptide chain. How are those polypeptide chains kept in an unfolded state following their synthesis in cytosol ?
During translation, a polypeptide chain is created using a RNA template. Which of the following components is responsible for bringing amino acids to the growing polypeptide chain? Multiple Choice o o o o O RNA polymerase o
The RNA meets with the organelle ___________________ that will read it to build a polypeptide chain of amino acids, following the process of ___________________. Specifically this organelle is attached on the ____________ ________________________which will help to modify and package the polypeptide product. It puts it into a transport vesicle to be sent over to the ___________________________ to add molecular tags and sort it, like a post office. This organelle puts the finished enzyme product inside a ____________________________ of phospholipids, which follows the network of...
The polypeptide chain of the chaperone protein GroEL from Escherichia colihas a molecular mass of 57200 Da and does not contain tryptophan or cystines. The A280of a 1 mg/mL solution of the purified protein in a 1 cm pathlength cuvette is 0.160. calculate the number of Tyr in the polypeptide chain.
Which of the following are needed for a ribosome to synthesize a polypeptide chain? aminoacyl-tRNAs ? RNA polymerase promoter ? helicase ???
Elongation of the polypeptide chain is terminated when a stop codon is incorporated into the newly synthesized protein. True False
In an enzyme, the polypeptide chain folds into a compact shape known as the structure, e quaternary secondary primary pleated tertiary