Question

OK. So, this week we’re investigating the eukaryotic gene PPtrl, which codes for the Rbbl protein. You painstakingly derived a segment of the transcribed sequence of this gene, which is listed below. What would be the mRNA sequence that corresponds with this segment?

coding

5’

A

T

G

C

G

T

A

A

T

G

C

T

A

3’

template

3’

T

A

C

G

C

A

T

T

A

C

G

A

T

5’

mRNA

5’

A

U

G

C

G

U

A

A

U

G

C

U

A

3’

1. OK. So, knowing what you do about this particular PPtrl gene, describe how the process of transcription initiation would go for this gene.
2. Would this RNA transcript be edited/processed prior to use in translation? How did you know that?

Answer 1

Like prokaryotes eukaryoute's RNA polymerase doesn't bind directly to the promoter it requires accessory proteins called transcription factors

Eukaryoutes have a promoter sequence called TATA box

The TATA box binding proteins bind to it with other transcrption factors

The first TF bind to TATA box is TFII D

Then TAB ( TATA binding proteins binds to it and stabilize it)

Then TFII A and TFII B bind then TFII E,F,H bind and form transcription pre initiation complex.

The unwinding of DNA by pre initiation complex called transcription initiation complex

2) yes ,the processing of m RNA is very important to protect it from​ nuclease activity as well as to make mRNA intron free.