Homework Answers
A cloning vector is a small piece of DNA that can be stably maintained in sn organism and into which a foreign DNA fragment can be inserted for cloning purposes.
An ideal cloning vector contains an origin of replication, a gene encoding resistance to an antibiotic, a multiple cloning site and a lacZ gene.
So, the answer is (c) a gene encoding reverse transcriptase.
Some vectors are designed for transcription only with no heterologous protein expressed, eg: for in-vitro mRNA production. These are called transcription vectors. They may lack the sequences necessary for the polyadenylation and termination. Therefore, it may not be used for protein production.
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An ideal cloning vector contains all of the following, EXCEPT an origin of replication. O Ob...
Cloning using a Simple Plasmid and Alpha Complementation
Which of the following characteristic is not present in a plasmid on a general basis?a) Multiple cloning site (MCS)b) Origin of replication (ori)c) Antibiotic resistance gened) Beta galactose genes
Foreign DNAs are inserted into a vector at a region called the? a. Anchor point b....
Foreign DNAs are inserted into a vector at a region called the? a. Anchor point b. Multiple Cloning Site c. Origin of replication d. Resistance gene e. Promoter region
(Choose the best answer) A polylinker increases the versatility of a DNA plasmid because it contains:...
(Choose the best answer) A polylinker increases the versatility of a DNA plasmid because it contains: a DNA recognition sequence for several different restriction enzymes. an antibiotic resistance cassette that allows selection in multiple different species. an origin of replication that is not organism-specific. a reverse transcriptase sequence. A BamHI restriction enzyme recognition sequence. cDNA libraries are the same as genomic libraries. include DNA from noncoding sequences. require DNA polymerase for their construction. require reverse transcriptase for their construction. are...
Question 2,3 and 4 aBbCcDdE AaBbCoDdE Normal No Spacing BamHI 300 EcoRI 3400 1000 2000 5 kb EcoRU Apal List three components a cloning vector must contain A cloning site A replication origin A sel...
Question 2,3 and 4
aBbCcDdE AaBbCoDdE Normal No Spacing BamHI 300 EcoRI 3400 1000 2000 5 kb EcoRU Apal List three components a cloning vector must contain A cloning site A replication origin A selectable marker -Drug resistant gene 1. 2. Draw the end of the fragment that will be generated after digestion with the Apal enzyme. Indicate the type of ends generated by this enzyme (Blunt/Sticky, 5' or 3' overhang) (3) 3. What is the size of the plasmid...
You wake up one morning to your roommate exclaiming about her sudden hair growth. She has...
You wake up one morning to your roommate exclaiming about her sudden hair growth. She has been supplementing her diet with a strange new fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the harE protein) that stimulates hair growth. You construct a fungal genomic DNA library in the hope of cloning the harЕ gene. If you succeed you will be a...
Below is a set of experiments for cloning the human growth hormone (HGH) gene and then...
Below is a set of experiments for cloning the human growth hormone (HGH) gene and then expressing the HGH protein in E. coli starting from human pituitary glands and a tube of plasmid vector DNA. The plasmid expression vector contains the arabinose inducible expression system. Specify the correct order for carrying out these experiments, using the letter codes in front of each procedure. Use all of the steps in your answer, except for one step that should NOT be included....
All of the following are true of poliomyeitis EXCEPT O initial sites of viral replication are...
All of the following are true of poliomyeitis EXCEPT O initial sites of viral replication are the throat and small intestine. most cases result in muscle paralysis. infection is due to ingestion of contaminated water the majority of cases are asymptomatic. the oral polio vaccine contains attenuated strains of the virus. O Previous Answers Request Answer SubmitPr X Incorrect; Try Again; 5 attempts remaining
please answer all amils A Doon E Conjagants DNA hips o sloning of freign DA fa...
please answer all
amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The...
Question 1: Saved Advantages of the Polymerase Chain Reaction include all of these, except: 1) The reaction is specific for certain sequences in the DNA. 2) Only small amounts of template are needed. 3) Results can only be obtained with freshly isolated DNA. 4) All the products from a specific part of the DNA will be the same size. Question 2: Saving The chain terminator used in Sanger's DNA sequencing is works due to.. 1) lack of 2'OH 2) ddNTP:...
Question 14 1 points Save Answer DNA replication poses several challenges to the cell that must...
Question 14 1 points Save Answer DNA replication poses several challenges to the cell that must be resolved for replication to proceed. All of the following are cellular solutions to the challenges posed by DNA replication except... O A. Helicase unwinds to the DNA to allow the replication fork to form. O B. Primase synthesizes an RNA primer since DNA polymerase can only extend an existing polynucleotide chain. O C. Higher eukaryotes utilize multiple origins of replication to reduce the...
Question 2,3 and 4
aBbCcDdE AaBbCoDdE Normal No Spacing BamHI 300 EcoRI 3400 1000 2000 5 kb EcoRU Apal List three components a cloning vector must contain A cloning site A replication origin A selectable marker -Drug resistant gene 1. 2. Draw the end of the fragment that will be generated after digestion with the Apal enzyme. Indicate the type of ends generated by this enzyme (Blunt/Sticky, 5' or 3' overhang) (3) 3. What is the size of the plasmid...
You wake up one morning to your roommate exclaiming about her sudden hair growth. She has been supplementing her diet with a strange new fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the harE protein) that stimulates hair growth. You construct a fungal genomic DNA library in the hope of cloning the harЕ gene. If you succeed you will be a...
All of the following are true of poliomyeitis EXCEPT O initial sites of viral replication are the throat and small intestine. most cases result in muscle paralysis. infection is due to ingestion of contaminated water the majority of cases are asymptomatic. the oral polio vaccine contains attenuated strains of the virus. O Previous Answers Request Answer SubmitPr X Incorrect; Try Again; 5 attempts remaining
please answer all
amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
Question 14 1 points Save Answer DNA replication poses several challenges to the cell that must be resolved for replication to proceed. All of the following are cellular solutions to the challenges posed by DNA replication except... O A. Helicase unwinds to the DNA to allow the replication fork to form. O B. Primase synthesizes an RNA primer since DNA polymerase can only extend an existing polynucleotide chain. O C. Higher eukaryotes utilize multiple origins of replication to reduce the...
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