Biochemistry
What would the effect be if the holoenzyme of DNA polymerase 3 of prokaryotes were defective?
Biochemistry What would the effect be if the holoenzyme of DNA polymerase 3 of prokaryotes were...
biochemistry 12 1.8 BCH2603/101/3/2020 The DNA polymerase involved in base excision repair is 1. DNA polymerase a 2. DNA polymerase B 3. DNA polymerase o 4. DNA polymerase v 5. DNA polymerase 1.9 Which of the following is true about phosphodiester linkage? 1.5 phosphate group of one nucleotide unit is joined to the 3-hydroxyl group of the next nucleotide 2.3-phosphate group of one nucleotide unit is joined to the 5-hydroxyl group of the next nucleotide De 0 DA
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is drawn incorrectly, they should be the same One is o pure onsa pyrimidine. c. One is a purine, one is a pyrimidine d. Double-stranded DNA is antiparallel. e. This is after DNA replication -before DNA replication, they look the same. 3....
RNA polymerase releases the DNA template. Initiation Elongation Termination A process called clearance or escape. The RNA polymerase holoenzyme binds to the promoter A process called clearance or escape. Reaching a terminator sequence causos formation of phosphodiester bonds to stop. The RNA polymerase holoenzyme is formed. Once bound to the promoter, RNA polymerase begins to unwind the DNA. New nucleotides are added to the 3' end of the growing RNA transcript. The RNA-DNA hybrid within the transcription bubble dissociates New...
Question 14 Which of the following is a feature common to BOTH prokaryotes and eukaryotes? The use of nucleosomes to condense DNA in the nucleus. The ability to translate an RNA before its transcription is complete. The ability to have multiple ribosomes on a single RNA for more efficient translation. The ability to start transcription at a 5'AUG sequence. o Question 15 A particular prokaryotic promoter contains only the region from-10 to-35. Which of the following is true? The RNA...
3. Specifically describe what would occur in DNA replication if the following components were missing, be thoughtful and do not simply state that DNA replication would not occur. Differentiate between prokaryotic and eukaryotic replication when appropriate. DNA ligase- DNA polymerase- Primer- Primase- Topoisomerase- Helicase- SSBP- Sliding clamp/PCNA- Telomerase-
Nick translation is an example of the process that would occur when DNA polymerase I is replacing an RNA primer with DNA. Which of these activities would be involved in nick translation? 3'-5' exonuclease and 5'-3' polymerase 5'-3' exonuclease and 5'-3' polymerase 5'-3' exonuclease and 3'-5' polymerase 3'-5' exonuclease and 3'-5' polymerase
What would be the effect of each type of the following mutations? a,) Mutation that destroys the function of eukaryotic DNA polymerase alpha. b.) A mutation that destroys the 5' to 3' exonuclease activity of DNA polymerase I.
What is the unique feature of A-DNA, B-DNA, and Z-DNA in Biochemistry?
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3' polymerase makes one misincorporation per 100,000 incorporations. A misincorporation occurs when the "wrong" nucleotide-residue is added to the growing DNA-molecule, such that the added residue is NOT complementary in the normal Watson-Crick way (G with C, A with T) to the residue in the template DNA. Normally, the 3'-to-5'exonuclease corrects most of these misincorporations, reducing the misincorporation rate to one in 10,000,000. If a...
*BIOCHEMISTRY QUESTION ABOUT MESELSON AND STAHL* There were 3 possible models for DNA replication prior to the Meselson and Stahl experiment, one of which was dispersive replication. a) Assuming that this is relatively uniform (each of the new chromosomes gets roughly equal amounts of the old ones) draw the peak traces for 0, 1, 2, 5 and 10 generations. b) If dispersive replication was not relatively uniform how would your answer change?