Question

D Asc D As c D As GR Vinc 2 hr 4 hr 6 hr CD A/D CDA/D C D A/D GR Vinc Figure 1: Western Blot of the effect of treatments on total and cytoplasmic glucocorticoid receptor (GR) protein levels. Cells were untreated (C), Dexamethasone (D), or Arsenic (As). A) Effect of treatments on total protein levels. B) Effect of treatments on cytoplasmic protein levels. Cell were treated with Arsenic 2-6 hr prior to Dexamethasone treatment (As/D). Con Dex As/Dex GEP

1. Looking at Figure 2:
   1. What question is being asked?

2. What is the main result?

Answer 1

Figure 2:

Question - What is the cellular localulocali of GR protein when treated with dexamethasone and arsenic compared to control cells?

Result - In control (untreated) cells, glucocorticoid receptor (GR) seems to be localized predominantly in the cytoplasm of the cells whereas when treated with dexamethasone and arsenic, GR is found to be present only in nucleus.

(When stained with both GFP and PI, which is a nuclear stain, the dexamethasone and arsenic images show yellow nucleus instead of red, which means the green of GFP and red of PI had combined to give yellow. Since green is not seen anywhere else, GR is present solely in nucleus in dexamethasone and arsenic treated cells)