What is the main take home result from Figure 1 part A?
What is the question that is being asked in figure 1 part B?
I need help with both questions please and can you explain how you got the answers.
Homework Answers
Result from figure 1 part A: When cells are treated with dexamethasone, total level of GR protein is reduced from 2 hour onwards. When cells are treated with arsenic, total level of GR protein reduces a bit after 6 hours of exposure, not before that.
Figure 1 part B: Question - What is the effect of dexamethasone and arsenic on cytoplasmic levels of GR protein under different periods of exposure (2, 4, 6 hrs)?
Add Answer to:
What is the main take home result
from Figure 1 part A?
What is the question...
Looking at Figure 2: What question is being asked? What is the main result? D Asc...
Looking at Figure 2:
What question is being asked?
What is the main result?
D Asc D As c D As GR Vinc 2 hr 4 hr 6 hr CD A/D CDA/D C D A/D GR Vinc Figure 1: Western Blot of the effect of treatments on total and cytoplasmic glucocorticoid receptor (GR) protein levels. Cells were untreated (C), Dexamethasone (D), or Arsenic (As). A) Effect of treatments on total protein levels. B) Effect of treatments on cytoplasmic protein levels....
I need help with a and b please. 4) Based on the image below (from Dean...
I need help with a and b please.
4) Based on the image below (from Dean et al 2001), answer the following questions (7). Serine/threonine protein phosphatase 5 (PP5) Control Nuclear > Cytoplasmic Localization of GR-GFP (%) ISIS 18321 Dermethasone Control Dexamethasone Dexamethasone Figure 2: Effects of ISIS 15534 and dexamethasone on the subcellular distribution of GR-GFP. A. Representative subcellular distribution of GR-GFP. A549 cells were grown and treated with ISIS 15521 (a mismatched control antisense oligonucleotide for ISIS 1535),...
Picture is at bottom. The figure of the western blot. Figure legend is also below that....
Picture is at bottom. The figure of the western blot. Figure
legend is also below that.
Question 7. (4 pt) The data shown in the figure below is from a study of the regulation of a protein called YB-1. a.) Based on the data in the figure, what mechanism of regulation is most likely induced by the treatments? b.) Briefly explain the evidence in the data that supports your conclusion about the mechanism. c.) What is the loading control in...
31. FGF receptor 1 (FGFR1) belongs to a family of tyrosine kinase receptors often amplified in...
31. FGF receptor 1 (FGFR1)
belongs to a family of tyrosine kinase receptors often amplified in
lung cancer. H520 cell line was stimulated by bFGF (20
ng/ml) for 20 min, with or without the pretreatment of AZD4547 (1
μM) for 40min. Then the cells were collected and analyzed by
Western blot (figure A). H520 cells were also stimulated
with bEGF (20 ng/ml) and protein levels were analyzed after 1, 6,
12, and 24 hours after bEGF treatment (Figure
B). Lastly, mRNA levels of...
Q 4. Explain how Fig.1A (arrow) result corroborates with the author hypothesis about Parkin aggre...
Q
4. Explain how Fig.1A (arrow) result corroborates with the author
hypothesis about Parkin aggresome and proteasome activity in PD?
MG-132+ HMW 127- 84-- 41- DTT+ IP T HMW 6- IP : anti-flag WB: anti-ubiquitin Fia. 1. Parkin forms plexes A, HEK 293Tells transiently tranafected with FLAG-Parkin were divided into twe dishes and were either untreated or treated with 5PM MG-132 for 161). Cells were then lysed abidใหr-taining 1% Triton X-100 ลnd fractitated into "Iuble (S) and insoluble u) frac...
2) Given that E6 inactivates p53-dependent pathways, cervical cancer cells are particularly resistant to traditional treatments...
2) Given that E6 inactivates p53-dependent pathways, cervical
cancer cells are particularly resistant to traditional treatments
such as chemotherapy, the search is ongoing for new alternative
therapeutics to be used in conjunction with current
chemotherapeutic drugs. One such candidate is the plant flavonoid
Luteolin. In 2014, Ham et al evaluate the effectiveness of Luteolin
on sensitizing cervical cancer cells to apoptosis.
a) In Figure 1, panel A, the researchers looked for an effect on
E6 levels post Luteolin treatment. What...
Total question is worth 10 marks A1. Answer ALL parts: In insulin-responsive cells the insulin receptor...
Total question is worth 10 marks A1. Answer ALL parts: In insulin-responsive cells the insulin receptor substrate 1 (IRS-1) protein is believed to be a major component of signal transduction. Several experiments were performed to investigate the relationship between IRS-1 and three adaptor proteins (Grb2, Shc, and p85) with respect to their role in insulin signaling. The descriptions of experiments are provided in the legends to the figures. Use Figures 1, 2 and 3 to provide the most appropriate answer....
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I am having difficulty...
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I
am having difficulty understanding the figures. I have copy and
pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated...
Please explain figures 6C, 6D, 6F, and 6G in simple terms! I am having difficulty understanding...
Please explain figures 6C, 6D, 6F, and 6G in simple
terms! I am having difficulty understanding the figures. I have
copy and pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated genes...
Identify the scientific premise, main hypothesis addressed by the paper and the research question of the...
Identify the scientific premise, main hypothesis addressed by
the paper and the research question of the paper
Also, what is the main results and the implication of
results
IMPORTANCE Clinical outcomes for glioblastoma remain poor. Treatment with immune checkpoint blockade has shown benefits in many cancer types. To our knowledge, data from a randomized phase 3 clinical trial evaluating a programmed death-1 (PD-1) inhibitor therapy for glioblastoma have not been reported. OBJECTIVE To determine whether single-agent PD-1 blockade with nivolumab...
Looking at Figure 2:
What question is being asked?
What is the main result?
D Asc D As c D As GR Vinc 2 hr 4 hr 6 hr CD A/D CDA/D C D A/D GR Vinc Figure 1: Western Blot of the effect of treatments on total and cytoplasmic glucocorticoid receptor (GR) protein levels. Cells were untreated (C), Dexamethasone (D), or Arsenic (As). A) Effect of treatments on total protein levels. B) Effect of treatments on cytoplasmic protein levels....
I need help with a and b please.
4) Based on the image below (from Dean et al 2001), answer the following questions (7). Serine/threonine protein phosphatase 5 (PP5) Control Nuclear > Cytoplasmic Localization of GR-GFP (%) ISIS 18321 Dermethasone Control Dexamethasone Dexamethasone Figure 2: Effects of ISIS 15534 and dexamethasone on the subcellular distribution of GR-GFP. A. Representative subcellular distribution of GR-GFP. A549 cells were grown and treated with ISIS 15521 (a mismatched control antisense oligonucleotide for ISIS 1535),...
Picture is at bottom. The figure of the western blot. Figure
legend is also below that.
Question 7. (4 pt) The data shown in the figure below is from a study of the regulation of a protein called YB-1. a.) Based on the data in the figure, what mechanism of regulation is most likely induced by the treatments? b.) Briefly explain the evidence in the data that supports your conclusion about the mechanism. c.) What is the loading control in...
31. FGF receptor 1 (FGFR1)
belongs to a family of tyrosine kinase receptors often amplified in
lung cancer. H520 cell line was stimulated by bFGF (20
ng/ml) for 20 min, with or without the pretreatment of AZD4547 (1
μM) for 40min. Then the cells were collected and analyzed by
Western blot (figure A). H520 cells were also stimulated
with bEGF (20 ng/ml) and protein levels were analyzed after 1, 6,
12, and 24 hours after bEGF treatment (Figure
B). Lastly, mRNA levels of...
Q
4. Explain how Fig.1A (arrow) result corroborates with the author
hypothesis about Parkin aggresome and proteasome activity in PD?
MG-132+ HMW 127- 84-- 41- DTT+ IP T HMW 6- IP : anti-flag WB: anti-ubiquitin Fia. 1. Parkin forms plexes A, HEK 293Tells transiently tranafected with FLAG-Parkin were divided into twe dishes and were either untreated or treated with 5PM MG-132 for 161). Cells were then lysed abidใหr-taining 1% Triton X-100 ลnd fractitated into "Iuble (S) and insoluble u) frac...
2) Given that E6 inactivates p53-dependent pathways, cervical
cancer cells are particularly resistant to traditional treatments
such as chemotherapy, the search is ongoing for new alternative
therapeutics to be used in conjunction with current
chemotherapeutic drugs. One such candidate is the plant flavonoid
Luteolin. In 2014, Ham et al evaluate the effectiveness of Luteolin
on sensitizing cervical cancer cells to apoptosis.
a) In Figure 1, panel A, the researchers looked for an effect on
E6 levels post Luteolin treatment. What...
Total question is worth 10 marks A1. Answer ALL parts: In insulin-responsive cells the insulin receptor substrate 1 (IRS-1) protein is believed to be a major component of signal transduction. Several experiments were performed to investigate the relationship between IRS-1 and three adaptor proteins (Grb2, Shc, and p85) with respect to their role in insulin signaling. The descriptions of experiments are provided in the legends to the figures. Use Figures 1, 2 and 3 to provide the most appropriate answer....
Please explain figures 6C, 6D, 6F, 6E, and 6G in simple terms! I
am having difficulty understanding the figures. I have copy and
pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated...
Please explain figures 6C, 6D, 6F, and 6G in simple
terms! I am having difficulty understanding the figures. I have
copy and pasted a section in the article. Thank you!!
Figure 6. MLL5 Suppresses Differentiation of GBM
Self-Renewing Cells
(A) Western blot to assess the effects of MLL5 knockdown in the
GBM primary culture G432NS.
(B) Western blots on cell lysates from two GBM cultures
differentiated over a 21-day period.
(C) Heatmap and unsupervised hierarchical clustering of
differentially regulated genes...
Identify the scientific premise, main hypothesis addressed by
the paper and the research question of the paper
Also, what is the main results and the implication of
results
IMPORTANCE Clinical outcomes for glioblastoma remain poor. Treatment with immune checkpoint blockade has shown benefits in many cancer types. To our knowledge, data from a randomized phase 3 clinical trial evaluating a programmed death-1 (PD-1) inhibitor therapy for glioblastoma have not been reported. OBJECTIVE To determine whether single-agent PD-1 blockade with nivolumab...
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