Homework Answers
* Function of enzyme in the ELISA:to show by a color change that the antigen is bound by the antibody
* Function of secondary antibody in the ELISA: to bind to the primary antibody.
* function of primary antibody in the ELISA: to bind to the primary antibody.
*source of antigen in direct ELISA : experimental sample w.g. human serum
function of antigen in indirect ELISA: to bind to the antibody in experimental sample.
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Match the ELISA reagent with its function. [ Choose) experimental sample (eg human serum) to bind...
ELISA 1. This ELISA method coats a 96 well plate with the antigen opium. If the...
ELISA 1. This ELISA method coats a 96 well plate with the antigen opium. If the patient's serum contains antibodies for opium, their primary antibody will bind to the antigen. Then a secondary conjugated antibody is used to bind to the primary antibody. What specific testing method, utilizes two types of antibodies? 2. Which ELISA method coats a 96 well plate with the antibodies that target THC? If the patient's serum contains the antigen THC, then they will test positive...
pls help, ty ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
Question 12 parts a,b,c Q12. Using the information provided below on ELISA, answer the following questions...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a...
You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice? A. Shelf life of reagents B. Speed of detection C. Wavelengths detected by microtiter plate reader D. Sensitivity Which assay format is the best choice for measuring small molecules, such as drugs and small peptide hormones? A....
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
Which electrolyte measurement is least affected by hemolysis? a. Calciumb. b- Magnesium c. Potassium d. Total...
Which electrolyte measurement is least affected by hemolysis? a. Calciumb. b- Magnesium c. Potassium d. Total Bilirubin Question 2 of 35 Required Question Which of the following should cause specimen rejection for platelet aggregation studies? a. The sample hematocrit is too high .b. The sample is hemolyzed. c. The sample is over-anticoagulated. d. The sample platelet count is too low. Question 3 of 35 Required Question Which of the following is usually positive in a patient with a urinary tract...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
ELISA 1. This ELISA method coats a 96 well plate with the antigen opium. If the patient's serum contains antibodies for opium, their primary antibody will bind to the antigen. Then a secondary conjugated antibody is used to bind to the primary antibody. What specific testing method, utilizes two types of antibodies? 2. Which ELISA method coats a 96 well plate with the antibodies that target THC? If the patient's serum contains the antigen THC, then they will test positive...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
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