Question

Home + Share a Insert Draw Arial BIU Design Layout References . 11 AAA Mallings >> LEE Paste XX A . S . A . C Styles TS Name: UTEP ID 1. Please list all the biochemical tests you performed in the Microbiology lab and what each test was looking for, including the main contents of the media and how they works (Please type your answers to this question and staple it to the back of this handout) 2 MSA is specifically used to isolate which type of bacteria? 3. What is the function of the enzyme catalase? 4. What is the oxidase tost used to reveal the presence of 5. Motility agar tests for the presence of what bacterial characteristic? 6. What type of media is commonly used to determine if an organism can use citrate as its sole carbon source Page 1 of 2 183 words Focus 3 - - + 124%

Answer 1

2. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens.
3. Catalase is a common enzyme, found in nearly all living organisms. It catalyses hydrogen peroxide into water and oxygen and protects organisms from free radicals It also has industrial uses to prevent certain contaminants in food and as a disinfectant for contact lenses and a cleansing agent in some other products.
-Catalase is constantly in battle against the effect of free radicals to the body. It transforms harmful superoxide radicals into hydrogen peroxide which later breaks down into water and oxygen.
4.cytochrome c oxidase test , The ability of Cytochrome C Oxidase to not only oxidize cytochrome c, but also Catalyze the Reduction of Cytochrome C by a Chromogenic Reducing Agent, which develops color as they become oxidized.
5.It is used to identify bacterial motility characters .
6. Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source.
7.Gram negative bacteria -MacConkey agar is a selective and differential culture media commonly used for the isolation of enteric Gram negative bacteria.because based on their ability to ferment lactose Bile salts .
-The Voges-Proskauer (VP) test is used to identify bacteria capable of 2,3 butanediol fermentation following mixed-acid fermentation .