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5. You have a bacteria that grows only the presence of oxygen, but you do not know if it is motile or not. What would you do

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Answer #1

Ans: Option C. can use either of them

Hanging drop method is a technique where a drop of bacterial culture is placed on a cavity microscope slide. A coverslip to which petroleum jelly is encircled around the edges, is used to cover the depression on which the drop of bacterial culture is present. This causes the bacterial culture to hang from the coverslip and the cavity slide is obderved under the microscope. Motile bacteria will move rapidly and non motile bacteria will remain still.

In semi solid motility agar method the bacterial culture is stabbed to the bottom of the test tube or inoculated in Petri plate containing semi solid media. Since the media is semi solid in nature, it allows the motile bacteria to move freely in the medium.

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