Question

If my gram staining test produce a gram negative short rods, what other test should I run to identify my bacteria?

Answer 1

Gram staining test already identify gram negative bacteria.

We will do the catalase test. By this test we can identify aerobic and anaerobic bacteria. Here we use H2O2 which can react with Catalase enzyme of bacteria and form O2 bubble. So if the O2 bubble is produced then we get the aerobic bacteria.

We will also do Methyl red test. By this test we can know which bacteria produce acid or which bacteria not. Here we use Methyl red , if the bacteria produce acid or acetoin then red color appear.