Question

I need help 1)Phagocytosis is a complex process that can be affected by many things. State...

I need help

1)Phagocytosis is a complex process that can be affected by many things. State a hypothesis as to something that you believe might affect phagocytosis in Tetrahymena.

2)What are the independent and dependent variables of your hypothesis?

3)What is a negative control you should use to rule out alternative explanations for a positive result?

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Answer #1

Aim: to determine the effect of pH on the process of phagocytosis on Tetrahymena

Hypothesis: Change in the pH from the neutral pH of 7, the rate of phagocytosis decreased.

Alternative hypothesis: there is no effect in the phagocytosis in Tetrahymena, with pH change.

Procedure:

· 5 tubes are taken with media for growth of Tetrahymena like nutrient media with 2% proteose-peptone.

· pH range of 5,6,7,8, 9 are maintained in each tube

· The time of incubation is kept constant for 2 hours.

· Number of phagocytotic vacuoles formed may be measured from each tube, for every 10 minutes for 2 hours.

Dependent variable: Number of phagocytotic vacuoles.

Independent variable: pH

Control: tube with pH 7

Interpretation:

The number of phagocytotic vacuoles decreases with change in pH. Thus, neutral pH (also acting as negative control) has the maximum number of phagocytotic vacuoles. This disproves the alternate hypothesis.

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