Question

1. Using the following DNA segment, the RNA transcribed and the translation DNA 5’ ATG CCG...

1. Using the following DNA segment, the RNA transcribed and the translation


DNA 5’ ATG CCG GGT ACA TTC GGC CCA TAC 3’
DNA 3’ TAC GGC CCA TGT AAG CCG GGT ATG 5’


RNA 5’ AUG CCG GGU ACA UUC GGC CCA UAC 3’
Protein met pro gly thr phe gly pro. tyr


DRAW an example of each of the following (circle the mutation that has occurred):
A silent mutation

A frameshift mutation

A nonsense mutation

A missense mutation

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Answer #1

silent mutation is a point mutation that does not change the amino acid, the original codon and the mutated codon codes for the same amino acid.

the 6th nucleotide is changed from G to T

mutated DNA is :
DNA 5’ ATG CCT GGT ACA TTC GGC CCA TAC 3’
DNA 3’ TAC GGA CCA TGT AAG CCG GGT ATG 5’

mutated RNA is : 5’ AUG CCU GGU ACA UUC GGC CCA UAC 3’

amino acid sequence is: Met-Pro-Gly-Thr-Phe-Gly-Pro-Tyr

A frameshift mutation is due to the insertion or deletion of nucleotides which are not in the multiples of 3, this changes the reading frame from that point.

C at the 18 th position is deleted

new DNA sequence

DNA 5’ ATG CCG GGT ACA TTC GGC CAT AC 3’
DNA 3’ TAC GGC CCA TGT AAG CCG GTA TG 5’

the new RNA is 5’ AUG CCG GGU ACA UUC GGC CAU AC 3’

the new amino acid seuquence is : Met-Pro-Gly-Thr-Phe-Gly-His-

Nonsense mutation is due to the point mutation which changes the codon to a stop codon.

the 24 th nucleotide is changed to G

the new DNA sequence is

DNA 5’ ATG CCG GGT ACA TTC GGC CCA TAG 3’
DNA 3’ TAC GGC CCA TGT AAG CCG GGT ATC 5’

the new RNA is : 5’ AUG CCG GGU ACA UUC GGC CCA UAG 3’

the amino acid sequence is Met-Pro-Gly-Thr-Phe-Gly-Pro

a Missense mutation changes the amino acid to another amino acid.

the fourth nucleotide is changed to A

the new DNA is

DNA 5’ ATG ACG GGT ACA TTC GGC CCA TAC 3’
DNA 3’ TAC TGC CCA TGT AAG CCG GGT ATG 5’


new RNA is

RNA 5’ AUG ACG GGU ACA UUC GGC CCA UAC 3’

the amino acid sequence is

Met-Thr-Gly-Thr-Phe-Gly-Pro-Tyr

the second amino acid is changed to Thr.

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