You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...

Question

Question

You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...

You need to perform a 5 x 10^-6 dilution. Give a detailed scheme of how you could carry out this dilution using serial dilutions similar to those you performed in class.

science biology

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

200 10019 Usb -- stock Tube Tube 2 e Tube 3 Tube 4 Tubes Pubes datokana 14e 1 Dilation - 1000 1000 050 1000 2001 S Torro 10 1 there is 8 tube taking into account stock.

First if you add 200 UL of stock solution to tube 1 which contain buffer media 800 UL . The solution will be diluted 1000/200 = 5 times.

All the calculation are shown in picture and then final dilution will be 5*10^-6.

Thank you

Add a comment

Answer 2

Similar Homework Help Questions

You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...

You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two...

Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two different approaches you can take (each using three steps/dilutions). Could someone please guide me through the steps of how to solve this dilution problem? I'm at a loss as to where to start
Draw out the serial dilution you will need to make using an original concentration of 10^8...

Draw out the serial dilution you will need to make using an original concentration of 10^8 and the Total Dilution Factors as 2, 4, 8, and 16.

i have 1.36x10^7 cells/mL and i need to perform a serial dilution to get 1.0x10^6/100 microliters....

i have 1.36x10^7 cells/mL and i need to perform a serial dilution to get 1.0x10^6/100 microliters. how is this done?
If you have 1.0 x 10 molecules in a tube and perform a 10-4 dilution, how...

If you have 1.0 x 10 molecules in a tube and perform a 10-4 dilution, how many molecules will be in your new tube? 1000 molecules 10,000 molecules • 10 molecules 100 molecules
Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml...

Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml (stock) and then prepared 1:100 dilutions as a working solution (tube A). The company also suggest verifying the optimal dilution for your experiment by doing serial (twofold) dilutions. Describe how you would prepare 500microliters of working solution (tube A). What volume of inhibitor will you add in microliter? 2) You would like to optimize the working concentration of the inhibitor, and therefore perform two-fold...

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
Now it's your turn to construct a dilution scheme to achieve a countable plate. You start...

Now it's your turn to construct a dilution scheme to achieve a countable plate. You start with a stock culture of 5.4 x 10^8 cells/mL. Draw a dilution scheme that will produce a countable plate (<300 colonies). Show how much is transferred at each step, how much media is in each flask or tube, and how much you will plate. Show how many colonies you expect on your final plate. Once you have constructed your scheme, answer this question: Is...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting...

#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting with tube 1 and ending in tube 9 with an initial concentration of 100 ug/mL tube # 1 2 3 4 5 6 7 8 9 10 [control] Kanamycin concentration (ug/ml) 100 ? ? ? ? ? ? ? ? 0 growth no no no no no yes yes yes yes yes #2) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw...

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...

You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...

Homework Answers

Add Answer to:
You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...

Post as a guest

Earn Coins

You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...

Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two...

Draw out the serial dilution you will need to make using an original concentration of 10^8...

i have 1.36x10^7 cells/mL and i need to perform a serial dilution to get 1.0x10^6/100 microliters....

If you have 1.0 x 10 molecules in a tube and perform a 10-4 dilution, how...

Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml...

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

Now it's your turn to construct a dilution scheme to achieve a countable plate. You start...

#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting...

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...

Homework Answers

Add Answer to: You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...

Post as a guest

Earn Coins

Add Answer to:
You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...