Question

Whats the source of siRNA for the regulation of mRNA expression and stability, please explain.

Answer 1

source of siRNA-- siRNA it is an exogenous double-stranded RNA.i 1998 Fire & Mello first established this in c. elegans.

stability of siRNA ---- lyophilized siRNA stable for one year at -20 degree in a freezer.

if siRNA is fluorescently labeled and stored in the dark it should be stable for six months at -20 degree

in the si RNA mediated RNAi pathway, the ds RNA is processed into siRNA duplexes comprises of two 21 nucleotide long strands with two nucleotides overhang at 3^' ends by an enzyme called dicer. the dicer includes an ATPase/rna helicase domain, catalytic RNase 3rd domain, and ds binding domain. dicer and ds RNA binding protein (together form the RISC complex) then load the RNA duplexes into RISC. the siRNA is thought to provide target specificity to RISC through the base pairing of the guide strand with the target mRNA.