what is the reason that simple stains will dye bacterial cells?
Homework Answers
Actually simple staining procedure incluid various steps.
one of them is fixation of cell. in this step we just make the sample immovable in order to study them properly, because purpose of simple staining is to study their structural part not about their movement.
So inorder to fix them (immobilize), here different grades of alcohol were used, which dehydrate the sample only to fix them at a particular stage. As there is dehydration of cell occurs, cell lost all the water content which ultimately cut all the connection of that cell with nature and cell(sample) become die.
Then there is application of colour (staining step). in case of simple staining mainly basic stains are used like methylene blue etc...
Basic stains are +vely charged so they tends to bonded with -vely charged alcohol treated sample (i.e. stains only attached to -vely charged part of bateria), which faciliates us to show the structural part of bacteria whereever the stains get attached.
SO THE SIMPLE STAIN WILL DYE BACTERIA ONLY BECAUSE OF OPPOSITE CHARGE INTERACTION WHICH FACILIATES STRONG INTERACTION BETWEEN STAIN AND BACTERIA.
THE BACTERIA GET DIE ONLY BECAUSE OF DEHYDRATION STEP &
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reason that bacterial cells use operons and polycistronic RNA.
Initiation of all genes in a biochemical pathway are regulated simultaneously. O operons are more stable than structural genes that are located singly on a chromosome. Bacterial cells have small genomes that cannot accommodate large numbers of regulatory regions. nscribing gene 0 Bacterial genes are very short, so RNA that is not polycistronic might be mistaken for fragments that should be...
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