Homework Answers
The answer is option E,becose the code that pair specific sequence of DND only .
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you explain and show work if possible. thank you.
21. Which primer pair would amplify...
2. Design a primer pair (5 nucleotides each) to amplify only exon 2 of the gene...
2. Design a primer pair (5 nucleotides each) to amplify only exon 2 of the gene model below. Use the primer pair you designed to model 3 cycles of DNA replication. 5..CGTGACTGATCATGGAGCTACGATCGACTCTCGAAGCTCTAGACGCTCTCGAGAGCTCTCGAATTAGATCGTAATTGCGGTTCA...3'
You want to use PCR to amplify the entire DNA shown in the figure below. Of...
You want to use PCR to amplify the entire DNA shown in the figure below. Of the listed primers, choose the pair that will allow you to amplify this stretch of DNA by PCR. (9 points total) DNA to be amplified 5’-CGATTCGAGCCATTCGAACTCGATCAGCCGATTCGATCAACCTTGGACAGTCAG-3’ 3’-GCTAAGCTCGGTAAGCTTGAGCTAGTCGGCTAAGCTAGTTGGAACCTGTCAGTC-5’ Primers: (1) 5’-GCTAAGCTCGGTA-3’ (5) 5’-CTTGGACAGTCAG-3’ (2) 5’-GAACCTGTCAGTC-3’ (6) 5’-CGATTCGAGCCAT-3’ (3) 5’-CTGACTGTCCAAG-3’ (7) 5’-ATGGCTCGAATCG-3’ (4) 5’-GACTGACAGGTTC-3’ (8) 5’-TACCGAGCTTAGC-3’ Answer: I will need primer number _____ and primer number _____
You are given the following double-stranded DNA template (only top strand shown). Design a primer-pair to...
You are given the following double-stranded DNA template (only top strand shown). Design a primer-pair to amplify all of the red (ds) sequence, and only the red sequence? Primers should be 8 nts long (note: usually 17-25 nts long) Hint: Think about direction of DNA synthesis and annealing of primer to double-stranded template ! To answer, write the primer sequence (8 nts each) into the provided space below with the indicated 5' 3' polarity. 5'---AATGCCGTCAGCCGATCTGCCTCGAGTCAATC GATGCTGGTAACTTGGGGTATAAAGCTTACCCATGG TATCGTAGTTAGATTGATTGTTAGGTTCTTAGGTTTA GGTTTCTGGTATTGGTTTAGGGTCTTTGATGCTATTA ATTGTTTGGTTTTGATTTGGTCTTTATATGGTTTATG TTTTAAGCCGGGTTTTGTCTGGGATGGTTCGTCTGAT...
You want to amplify the DNA between the two stretches of sequence shown below. Of the...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5'-ACATTACCAA----GACCTGCTTT-3' 3' -TGTAATGGTT----CTGGACGAAA-5' 5' -TGTAATGGTT-3' and 5'-TTTCGTCCAG-3' O 5'-ACATTACCAA-3' and 5'-AAAGCAGGTC-3' 5'-TGTAATGGTT-3' and 5'-CTGGACGAAA-3' O 5'-AACCATTACA-3' and 5'-CTGGACGAAA-3'
You want to amplify the DNA between the two stretches of sequence shown below. Of the...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5’-CTGGACGAAA----TGTAATGGTT-3’ 3’-GACCTGCTTT----ACATTACCAA-5’ A. 5’-CTGGACGAAA-3’ and 5’-TGTAATGGTT-3’ B. 5’-CTGGACGAAA-3’ and 5’-AACCATTACA-3’ C. 5’-TTTCGTCCAG-3’ and 5’-TGTAATGGTT-3’ D. 5’-GACCTGCTTT-3’ and 5’-ACATTACCAA-3’
Can you answer and explain it please Q. You want to PCR amplify the underlined/bold DNA...
Can you answer and explain it please
Q. You want to PCR amplify the underlined/bold DNA fragment. Design two primers for this job. The size of each primer is 18 bp -3 Forward primer: 5'- Reverse primer: 5'-( ATGACTATAGGGAACACTACGAGTGTGACAACGGTACGTAAC GCGGCGATCGAGCG ACCGAA GCTACTCCGCCTTTAGCTCTCGACAAT ATCGACGCCGCTA CGGATGTGTTTAGGCCTGGT GA TGCTGG AACCCTTTA TTCGCCGAGGAAG ATGTTATCAGCGGGTCTATCAGCATCA CGATCATCGTGAGCT GGTTCTTGGCTATTTCGGAGCAGGT GTCGAATCCGATGCT ITTGTGGGTGAGATGGGTTTATTCA GTGAAGTC ATCTTACGTACACGGA ACTCAGTGTGAATTGG AAGGC AGTTTAGCGAGTGATGCGCCGCGGATTTTGTATGCGATAGGTAT GCAGTTATCTAAGCGTTTATTGCTTACCAC
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence...
Pick a pair of primers that you could use to amplify the
following double-stranded DNA sequence using PCR. Note: Both
strands shown below.
5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3'
3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5'
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence using PCR. Note: Both strands shown below 5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3 3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5 5'-CCACTAG-3'; 5'-CGTTAAT-3' 5'-GGTGATC-3'; 5'-GCAATTA-3' 5'-GATCACC-3':5'-CGTTAAT-3' 5'-CCACTAG-3';5'-GCAATTA-3' 5'-GGTGATC-3';5'-CGTTAAT-3'
"You wish to amplify the region of DNA between the bolded nucleotides below. Determine the REVERSE...
"You wish to amplify the region of DNA between the bolded nucleotides below. Determine the REVERSE primer that you will use for PCR. Write the primer sequence 5` to 3` with no spaces. Make the primer 10 nucleotides long. 5` AGTCA CGATG TTCGC TAGAT AGCTC GATAT ATTTG CCGAT CGCCT 3`
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence...
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence using PCR. Note: Both strands shown below. 5'-GGTGATCGGAGCTACGTAGGCACGGCATTACACGGATAGGCTAATTGC-3' 3'-CCACTAGCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGATTAACG-5' 5'-CCACTAG-3' ; 5'-GCAATTA-3' 5'-GATCACC-3' ; 5'-CGTTAAT-3' 5'-GGTGATC-3' ; 5'-GCAATTA-3' 5'-CCACTAG-3' ; 5'-CGTTAAT-3' 5'-GGTGATC-3' ; 5'-CGTTAAT-3'
Which of the following primer sets would be capable of generating an amplicon consisting of nucleotides...
Which of the following primer sets would be capable of
generating an amplicon consisting of nucleotides only found in the
bracketed region?
Note: More than may answer can be selected
a.
5’-GAC-3’ and 5’-ATA3’
b.
5’-TAT-3’ and 5’-CTG-3’
c.
5’-TAT-3’ and 5’-GTC-3’
d.
5’-TCA-3’ and 5’-TTA-3’
e.
5’-ATA-3’ and 5’-TAT-3’
Suppose you want to amplify the following bracketed sequence. — 3'-AATCG [TATCAGCAGCAGCAG]TGACT-5 – to - 5'-ITAGC[ATAGICGICGICGTC]ACIGA-3
2. Design a primer pair (5 nucleotides each) to amplify only exon 2 of the gene model below. Use the primer pair you designed to model 3 cycles of DNA replication. 5..CGTGACTGATCATGGAGCTACGATCGACTCTCGAAGCTCTAGACGCTCTCGAGAGCTCTCGAATTAGATCGTAATTGCGGTTCA...3'
You are given the following double-stranded DNA template (only top strand shown). Design a primer-pair to amplify all of the red (ds) sequence, and only the red sequence? Primers should be 8 nts long (note: usually 17-25 nts long) Hint: Think about direction of DNA synthesis and annealing of primer to double-stranded template ! To answer, write the primer sequence (8 nts each) into the provided space below with the indicated 5' 3' polarity. 5'---AATGCCGTCAGCCGATCTGCCTCGAGTCAATC GATGCTGGTAACTTGGGGTATAAAGCTTACCCATGG TATCGTAGTTAGATTGATTGTTAGGTTCTTAGGTTTA GGTTTCTGGTATTGGTTTAGGGTCTTTGATGCTATTA ATTGTTTGGTTTTGATTTGGTCTTTATATGGTTTATG TTTTAAGCCGGGTTTTGTCTGGGATGGTTCGTCTGAT...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5'-ACATTACCAA----GACCTGCTTT-3' 3' -TGTAATGGTT----CTGGACGAAA-5' 5' -TGTAATGGTT-3' and 5'-TTTCGTCCAG-3' O 5'-ACATTACCAA-3' and 5'-AAAGCAGGTC-3' 5'-TGTAATGGTT-3' and 5'-CTGGACGAAA-3' O 5'-AACCATTACA-3' and 5'-CTGGACGAAA-3'
Can you answer and explain it please
Q. You want to PCR amplify the underlined/bold DNA fragment. Design two primers for this job. The size of each primer is 18 bp -3 Forward primer: 5'- Reverse primer: 5'-( ATGACTATAGGGAACACTACGAGTGTGACAACGGTACGTAAC GCGGCGATCGAGCG ACCGAA GCTACTCCGCCTTTAGCTCTCGACAAT ATCGACGCCGCTA CGGATGTGTTTAGGCCTGGT GA TGCTGG AACCCTTTA TTCGCCGAGGAAG ATGTTATCAGCGGGTCTATCAGCATCA CGATCATCGTGAGCT GGTTCTTGGCTATTTCGGAGCAGGT GTCGAATCCGATGCT ITTGTGGGTGAGATGGGTTTATTCA GTGAAGTC ATCTTACGTACACGGA ACTCAGTGTGAATTGG AAGGC AGTTTAGCGAGTGATGCGCCGCGGATTTTGTATGCGATAGGTAT GCAGTTATCTAAGCGTTTATTGCTTACCAC
Pick a pair of primers that you could use to amplify the
following double-stranded DNA sequence using PCR. Note: Both
strands shown below.
5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3'
3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5'
Pick a pair of primers that you could use to amplify the following double-stranded DNA sequence using PCR. Note: Both strands shown below 5'-CCACTAGGGAGCTACGTAGGCACGGCATTACACGGATAGGCATTAACG-3 3'-GGTGATCCCTCGATGCATCCGTGCCGTAATGTGCCTATCCGTAATTGC-5 5'-CCACTAG-3'; 5'-CGTTAAT-3' 5'-GGTGATC-3'; 5'-GCAATTA-3' 5'-GATCACC-3':5'-CGTTAAT-3' 5'-CCACTAG-3';5'-GCAATTA-3' 5'-GGTGATC-3';5'-CGTTAAT-3'
Which of the following primer sets would be capable of
generating an amplicon consisting of nucleotides only found in the
bracketed region?
Note: More than may answer can be selected
a.
5’-GAC-3’ and 5’-ATA3’
b.
5’-TAT-3’ and 5’-CTG-3’
c.
5’-TAT-3’ and 5’-GTC-3’
d.
5’-TCA-3’ and 5’-TTA-3’
e.
5’-ATA-3’ and 5’-TAT-3’
Suppose you want to amplify the following bracketed sequence. — 3'-AATCG [TATCAGCAGCAGCAG]TGACT-5 – to - 5'-ITAGC[ATAGICGICGICGTC]ACIGA-3
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