Will the BCA Assay allow you to determine the concentration of LDH specifically or the total...
Will the BCA Assay allow you to determine the concentration of LDH specifically or the total protein concentration in your sample? Explain your answer.
Homework Answers
No, BCA assay will not allow you to determine the concentration of LDH specifically rather it allows to determine the total protein concentration in the sample.
The principle of this assay include the reaction between protein and Cu2+ ion. The protein reduce the Cu2+ ion into Cu+ ion in alkaline medium which forms purple color with bicinchoninic acid. The total protein concentration is determined by color change of the sample solution from green to purple in relation to protein concentration. The intensity of the color is measured by using colorimetric techniques.
Since, color change depends on total protein concentration it is not specific to LDH only.
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Will the BCA Assay allow you to determine the concentration of
LDH specifically or the total...
You are given 300 µl of LDH and are told that it has a total activity...
You are given 300 µl of LDH and are told that it has a total activity of 25 U. The concentration of protein in this sample is 10 mg/ml. Calculate this sample’s enzyme concentration in units of U/ml. Calculate also the specific activity of this sample in units of U/mg.
In my lab, we ran a BCA assay for protein concentration and generated this standard curve,...
In my lab, we ran a BCA assay for protein concentration and
generated this standard curve, but I have absolutely no idea what
this chart is telling me. Please explain if you can! Thank you.
Curve 3.000 2.500+ 2.000 562 1.500+ 1.000 0.500 0.000+ -500 500 1500 2000 2500 1000 <Plate Layout Settings R2 Curve Curve Name Formula Curve Y=A*X+B Curve Curve Paramet Name Formula er Curve Y=A*X+B A B Fit F Prob 0.00101 0.2020.995 ????? Std. 95% CI 95%...
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
While performing the assays for the LDH kinetics experiment, you will pipet 25ul of 250 ug/ml...
While performing the assays for the LDH kinetics experiment, you will pipet 25ul of 250 ug/ml LDH into 975 ul of assay buffer (Tris, Lactate, and NAD). What is the final concentration of LDH in the assay? I am having trouble if the V2 in M1V1=M2V2 is either 975 ul or if its 1000 ul
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent ...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1...
Pre
lab quiz question
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the sample for a tube using 0.6 mL of the 2.0 mg/ml standard BSA Your Answer: Answer units
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating...
In my lab, we ran a BCA assay for protein concentration and
generated this standard curve, but I have absolutely no idea what
this chart is telling me. Please explain if you can! Thank you.
Curve 3.000 2.500+ 2.000 562 1.500+ 1.000 0.500 0.000+ -500 500 1500 2000 2500 1000 <Plate Layout Settings R2 Curve Curve Name Formula Curve Y=A*X+B Curve Curve Paramet Name Formula er Curve Y=A*X+B A B Fit F Prob 0.00101 0.2020.995 ????? Std. 95% CI 95%...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Pre
lab quiz question
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the sample for a tube using 0.6 mL of the 2.0 mg/ml standard BSA Your Answer: Answer units
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating...
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