The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal translocation (movement of the ribosome relative to...
The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal translocation (movement of the ribosome relative to the mRNA). It does not affect any other step in protein synthesis. Suppose you are doing a translation reaction in vitro and you add erythromycin just before the 3rd peptide bond is about to be made. You wait a few minutes and look at the ribosome.
Circle the correct answer:
a. The carboxyl end of AA #3 should now be covalently bonded directly to: (tRNA #2) (tRNA #3) (tRNA #4) (AA #2) (AA #3) (AA #4) (none of these).
b. Which tRNA will you find in the A site? (tRNA #1) (tRNA #2) (tRNA #3) (tRNA #4) (tRNA #5).
c. The tRNA in the A site will carry: (an amino acid) (a polypeptide) (neither) (either one)
Homework Answers
a. The carboxyl end of AA #3 should now be covalently bonded directly to AA # 4 because erythromycin do not inhibit the process of peptide bond formation, it inhibits the translocation of mRNA along ribosome
b. In the A site, we will find tRNA # 3
c. Always tRNA in the A site will carry amino acid only, the tRNA at P site only carry the polypeptide.
Add Answer to:
The antibiotic erythromycin disrupts protein synthesis by
preventing ribosomal translocation (movement of the ribosome
relative to...
Multiple choice questions. Please highlight, circle answers, or make them obvious when displayed alongside explanations. (5...
Multiple choice questions. Please highlight, circle answers, or make them obvious when displayed alongside explanations. (5 pts) The antibiotic erythromycin disrupts protein synthesis by preventing ribosomal translocation (movement of the ribosome relative to the mRNA). It does not affect any other step in protein synthesis. Suppose you are doing a translation reaction in vitro and you add erythromycin just before the 3rd peptide bond is about to be made. You wait a few minutes and look at the ribosome. Circle...
Tetracycline inhibits protein synthesis by which mechanism? It interacts with the small ribosomal subunit, blocking access...
Tetracycline inhibits protein synthesis by which mechanism? It interacts with the small ribosomal subunit, blocking access of the aminoacyl-tRNA to the mRNA-ribosome complex. It binds to the 305 subunit and distorts its structure, interfering with the initiation of protein synthesis. It binds irreversibly to a site on the 50S subunit of the bacterial ribosome, thus inhibiting translocation. It inactivates the eukaryotic elongation factor, eEF-2, thus preventing translocation.
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a...
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
QUESTION 15 Antibiotics have been very useful in elucidating the steps of protein synthesis. If you...
QUESTION 15 Antibiotics have been very useful in elucidating the steps of protein synthesis. If you have an artificial mRNA with the sequence 5'-AUGGGGGGGGGG..., it will produce the following peptide in a cell-free, protein synthesizing system: fMet-Gly-Gly-Gly-Gly.... In your search for new antibiotics, you find one that blocks protein synthesis. When the same artificial mRNA is added to the cell free system with the antibiotic, only the dipeptide product: fMet-Gly is produced. Which step in protein synthesis could this antibiotic...
Table 1B: Protein Synthesis with 2nd DNA Template Strand DNA Codons in the 2nd Template Strand...
Table 1B: Protein Synthesis with 2nd DNA Template Strand DNA Codons in the 2nd Template Strand mRNA Sequence (List codons) Amino Acids in the Protein **Use the Genetic Code Chart on page 217 to determine the amino acids that will be placed in the protein Questions: 19. The three letter "code words of DNA and RNA that specify amino acids are called: A. codons B. promoters C. Introns D. anticodons 20. Proteins are composed of building blocks called: A. fatty...
5) Translation was originally sorted out using a variety of inhibitors of protein synthesis Inhibitors tend...
5) Translation was originally sorted out using a variety of inhibitors of protein synthesis Inhibitors tend to interrupt the process at a particular step and a population of translating ribosomes will all stop in the same state. An allied technique is to identify temperature sensitive mutations in various accessory factors. When the population of ribosomes is shifted to the restrictive temperature, ribosomes will stall when they need the function of the mutant protein to continue By analyzing the structure of...
3. What are the “translator” molecules that recognize a codon in the mRNA and deliver the correct...
3. What are the “translator” molecules that recognize a codon in the mRNA and deliver the correct amino acid? 6. If each amino acid was encoded by a single codon, what is the minimum number of amino-acyl tRNA synthetases required for translation? 7. Looking at the codon table, if there was a unique aminoacyl-tRNA synthetase required for each anticodon, what is the minimum required? 9. If an aminoacyl-tRNA synthetase recognized any nucleotide (purine or pyrimidine) in the 5’end of the anticodon,...
Please use the drop down menu of answer choices to answer all questions. Reference both picturea...
Please use the drop down menu of answer choices to answer all
questions. Reference both picturea for all possible answer choices.
Thanks!
1. At this step in the process of translation the ribosome assemble around the mRNA 2 At this step in translation the tRNA takes amino acids to the ribosomes attached to mRNA_to build a polypeptide chain 3. At this step in translation the ribosome releases the polypeptide chain 4. True/False in prokaryotes translation takes place in the cytoplasm...
answer all the questions 18) A mutation occurs such that a spliceosome cannot remove one of...
answer all the questions
18) A mutation occurs such that a spliceosome cannot remove one of the introns in a gene. What effect will this have on the gene? Translation will continue, but a nonfunctional protein will be made b) Translation will continue and will skip the intron sequence c) It will have no effect; the gene will be transcribed and translated into protein d) Transcription will terminate easily and the protein will not be made 19. During the process...
3. (4 pts) Puromycin is an antibiotic that has an effect on both prokaryotes and eukaryotes....
3. (4 pts) Puromycin is an antibiotic that has an effect on both prokaryotes and eukaryotes. The drug, which is structurally similar to the aminoacyl terminus of an aminoacyl-tRNA (see figure below), inhibits protein synthesis by releasing nascent polypeptide chains before their synthesis is completed. н,с CH NH2 N N N CH CH HC нс. N N O 'N HOCH 0 R-O-POCHO H H 0 H H H H H H HAN OH O OH CO C=0 H-C-CH2 OCH H-C-R...
Tetracycline inhibits protein synthesis by which mechanism? It interacts with the small ribosomal subunit, blocking access of the aminoacyl-tRNA to the mRNA-ribosome complex. It binds to the 305 subunit and distorts its structure, interfering with the initiation of protein synthesis. It binds irreversibly to a site on the 50S subunit of the bacterial ribosome, thus inhibiting translocation. It inactivates the eukaryotic elongation factor, eEF-2, thus preventing translocation.
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
QUESTION 15 Antibiotics have been very useful in elucidating the steps of protein synthesis. If you have an artificial mRNA with the sequence 5'-AUGGGGGGGGGG..., it will produce the following peptide in a cell-free, protein synthesizing system: fMet-Gly-Gly-Gly-Gly.... In your search for new antibiotics, you find one that blocks protein synthesis. When the same artificial mRNA is added to the cell free system with the antibiotic, only the dipeptide product: fMet-Gly is produced. Which step in protein synthesis could this antibiotic...
Table 1B: Protein Synthesis with 2nd DNA Template Strand DNA Codons in the 2nd Template Strand mRNA Sequence (List codons) Amino Acids in the Protein **Use the Genetic Code Chart on page 217 to determine the amino acids that will be placed in the protein Questions: 19. The three letter "code words of DNA and RNA that specify amino acids are called: A. codons B. promoters C. Introns D. anticodons 20. Proteins are composed of building blocks called: A. fatty...
5) Translation was originally sorted out using a variety of inhibitors of protein synthesis Inhibitors tend to interrupt the process at a particular step and a population of translating ribosomes will all stop in the same state. An allied technique is to identify temperature sensitive mutations in various accessory factors. When the population of ribosomes is shifted to the restrictive temperature, ribosomes will stall when they need the function of the mutant protein to continue By analyzing the structure of...
Please use the drop down menu of answer choices to answer all
questions. Reference both picturea for all possible answer choices.
Thanks!
1. At this step in the process of translation the ribosome assemble around the mRNA 2 At this step in translation the tRNA takes amino acids to the ribosomes attached to mRNA_to build a polypeptide chain 3. At this step in translation the ribosome releases the polypeptide chain 4. True/False in prokaryotes translation takes place in the cytoplasm...
answer all the questions
18) A mutation occurs such that a spliceosome cannot remove one of the introns in a gene. What effect will this have on the gene? Translation will continue, but a nonfunctional protein will be made b) Translation will continue and will skip the intron sequence c) It will have no effect; the gene will be transcribed and translated into protein d) Transcription will terminate easily and the protein will not be made 19. During the process...
3. (4 pts) Puromycin is an antibiotic that has an effect on both prokaryotes and eukaryotes. The drug, which is structurally similar to the aminoacyl terminus of an aminoacyl-tRNA (see figure below), inhibits protein synthesis by releasing nascent polypeptide chains before their synthesis is completed. н,с CH NH2 N N N CH CH HC нс. N N O 'N HOCH 0 R-O-POCHO H H 0 H H H H H H HAN OH O OH CO C=0 H-C-CH2 OCH H-C-R...
Most questions answered within 3 hours.
-
A combustion reaction is describes as a carbon source reacting
with oxygen and producing carbon dioxide...
asked 6 minutes ago -
Buckminsterfullerence is a recently allotrope of carbon in which
carbon atoms form molecules of formula C_60,...
asked 8 minutes ago -
Lower Equitorial and Upper Equitorial are the same except Lower
Equitorial has a larger capital stock....
asked 13 minutes ago -
how do you think that pH of a jar where you have added a certain
amount...
asked 23 minutes ago -
If the Federal Reserve increases the reserve requirement, what
will happen to the Money Supply in...
asked 18 minutes ago -
Suppose that market demand for a good is given by Q = 9 - 0.3 P...
asked 24 minutes ago -
two thin lenses are separated by a distance x. The first lens
has a focal length...
asked 25 minutes ago -
The computer that controls a bank's automatic teller machine
crashes a mean of 0.6 times per...
asked 29 minutes ago -
`1) How is -9 (base 10) represented in 8-bit two's complement
notation?
a) 00001001
b)11110111
c)11110110...
asked 39 minutes ago -
A 10.000 g sample of water contains 11.19% H by mass. what
should be the %H...
asked 56 minutes ago -
Consider an investment game among 2 players. Each player can
either invest,
i, or not invest,-i....
asked 54 minutes ago -
The time taken to complete a particular task is normally
distributed with a standard deviation of...
asked 1 hour ago