Homework Answers
B. GFP expression allows specific cells to be easily observed.
It is a very important scientific tool. It is used as a visual tag for expressing other genes. It is a marker protein.
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Question 1 of 40 What is the advantage of using GFP as a marker in a...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
I posted this question before but no answer I need to submit in 4 hours, please...
I posted this question before but no answer I need to submit in
4 hours, please help.
Although the following scenario is hypothetical, it is based on actual facts. Sickle cell anemia is an inherited form of anemia. Those with sickle cell anemia do not have enough healthy red blood cells to adequately carry oxygen to all the cells in their body. Normal red blood cells are flexible and round, and can easily move through the blood vessels. However, in...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
Using the HCAPHS patient satisfaction survey questions 1-4, 11, 13, 14, 17, identify & explain 2...
Using the HCAPHS patient satisfaction survey questions 1-4, 11,
13, 14, 17, identify & explain 2 nursing specific strategies
that nurses can implement to improve the patient experience. Use
information that you have observed in clinical area and what you
have researched. Must have citations and references-see
instructions.
OVERALL RATING OF HOSPITAL 5. YOUR CARE FROM DOCTORS During this hospital stay, how often did doctors treat you with courtesy and respect? ' Never 20 Sometimes ' Usually 40 Always YOUR...
8-Using the HCAPHS patient satisfaction survey questions 1-4, 11, 13, 14, 17, identify & explain 2...
8-Using the HCAPHS patient satisfaction
survey questions 1-4, 11, 13, 14, 17, identify & explain 2
nursing specific strategies that nurses can implement to improve
the patient experience. Use information that you have observed in
clinical area and what you have researched. Must have
citations and references-see instructions.
HCAHPS Survey SURVEY INSTRUCTIONS • You should only fill out this survey if you were the patient during the hospital stay named in the cover letter. Do not fill out this survey...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
What is the correct modifier to use for the following three operative reports Assignment #1 PROCEDURE...
What is the correct modifier to use for the following three operative reports Assignment #1 PROCEDURE PERFORMED: Cataract extraction with lens implantation, right eye. DESCRIPTION OF PROCEDURE: The patient was brought to the operating room. The patient was identified and the correct operative site was also identified. A retrobulbar block using 5 ml of 2% lidocaine without epinephrine was done after adequate anesthetic was assured, and the eye was massaged to reduce risk of bleeding. The patient was prepped and...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
I posted this question before but no answer I need to submit in
4 hours, please help.
Although the following scenario is hypothetical, it is based on actual facts. Sickle cell anemia is an inherited form of anemia. Those with sickle cell anemia do not have enough healthy red blood cells to adequately carry oxygen to all the cells in their body. Normal red blood cells are flexible and round, and can easily move through the blood vessels. However, in...
Using the HCAPHS patient satisfaction survey questions 1-4, 11,
13, 14, 17, identify & explain 2 nursing specific strategies
that nurses can implement to improve the patient experience. Use
information that you have observed in clinical area and what you
have researched. Must have citations and references-see
instructions.
OVERALL RATING OF HOSPITAL 5. YOUR CARE FROM DOCTORS During this hospital stay, how often did doctors treat you with courtesy and respect? ' Never 20 Sometimes ' Usually 40 Always YOUR...
8-Using the HCAPHS patient satisfaction
survey questions 1-4, 11, 13, 14, 17, identify & explain 2
nursing specific strategies that nurses can implement to improve
the patient experience. Use information that you have observed in
clinical area and what you have researched. Must have
citations and references-see instructions.
HCAHPS Survey SURVEY INSTRUCTIONS • You should only fill out this survey if you were the patient during the hospital stay named in the cover letter. Do not fill out this survey...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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