Question

How do you prepare a 6 mM solution of zinc chloride in 0.1 M MOPS buffer?...

How do you prepare a 6 mM solution of zinc chloride in 0.1 M MOPS buffer? How do you know how much buffer to put in the solution for the desired final concentration? Same question for preparing a 0.4 mM solution of a peptide with molecular weight of 1565.8 g/mol in 0.1 M MOPS? I dont understand how the buffer can have a different concentration than final solution but be used to make the solution?

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Answer #1

In this question you need two solutions, 0.1 M MOPS and 6 mM ZnCl.

So, in distilled water you will add MOPS and ZnCl according to the required weight.

O-I M MOP 6mM for X 136 000 IL) (duppose volun o be pupared 6X 136 (O0 MOOS Z09 gn 500 me diit- walr Not MOPS Shae E dselue M

Similarly, you can prepare the other solution. Instead of ZnCl, take 0.4 mM of peptide.

If you have any doubt then you can ask me in the comment section.

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