In PCR, how is the segment of DNA that is to be amplified determined? A. By...
In PCR, how is the segment of DNA that is to be amplified determined?
A. By the activity of the polymerase
B. By the extension of the annealed primers
C. By using primers that flank the target sequence
D. By denaturation of the entire genome
Homework Answers
In PCR the segment of DNA that is to be amplified is determined by using primers that flank the target sequence
Explanation :- in PCR targeted sequence are amplified by using primer, primers are known sequence of DNA which attached to targeted sequence by complementary mean.after attachment of primer to target sequence Taq Polymerase binds to DNA and complete replication/ amplification of target sequence.
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In PCR, how is the segment of DNA that is to be amplified
determined?
A. By...
Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA...
Which of the following statements about the polymerase chain reaction (PCR) is false? a The DNA ligase used is from a thermophilic bacteria so that it can resist denaturation at high temperatures. b Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. c The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis. d DNA amplified by PCR can be cloned.
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts...
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts of a target DNA sequence to be amplified. b) two primers, complementary to each end of the target sequence. c) a large supply of DNA nucleotides. d) a heat-stable DNA polymerase. e) All of the above
Which of the following is needed for the PCR technique to work? (multiple answers) a. A...
Which of the following is needed for the PCR technique to work? (multiple answers) a. A probe that can anneal to the center of the target DNA sequence. b. A thermostable RNA polymerase c. Large amounts of pure DNA d. A thermostable DNA polymerase e. Two DNA primers that can anneal to the target DNA on either side of the DNA to be amplified. f. A thermostable DNA ligase
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
PCR utilizes specific DNA primers and polymerase enzyme to make copies of particular DNA sequence. The...
PCR utilizes specific DNA primers and polymerase enzyme to make copies of particular DNA sequence. The primers must attach to separated DNA at the target sequences so that the polymerase can build new DNA strands. What must also be added to the mixture besides primers and polymerase enzyme in order to get amplification and DNA? a. RNA polymerase b. dNTPs c. electron carriers d. DNA repair enzymes
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)?...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A....
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A. Make lots of copies of a specific DNA sequence to use in cloning (preparative PCR) B. Detect the presence or relative amount of a specific gene under varying conditions (analytical PCR) *Remember: PCR is very similiar to DNA replication, but uses a DNA polymerase to amplify only specific parts of DNA sequence based on the sequence of the primers. 3. For which goal (A...
DNA from 100 unrelated people was amplified by PCR with allele specific primers, The PCR products...
DNA from 100 unrelated people was amplified by PCR with allele specific primers, The PCR products were separated and visualized on the following gel. A specific fragment was found to correspond to each blood group allele, indicated on the left hand side. The number of individuals with each gel pattern is shown across the top. 7351-749, 18 Assuming random mating, what is the expected frequency of Type B blood? А 0.0025 0.070 C. 0.0725 D 0.25 E 0.05
Which of the following components is NOT used in the technique of PCR to amplify a...
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
The DNA primers used in PCR are a. complementary to DNA sequences at both ends of...
The DNA primers used in PCR are a. complementary to DNA sequences at both ends of the DNA sequence of interest. b. attached to the gene of interest by ligase. c. produced when a gene of interest is read by restriction enzymes. d. identical to the entire base sequence of one strand of the DNA.
Question 33 (2 points) The requirements of the polymerase chain reaction (PCR) include: a) knowing parts of a target DNA sequence to be amplified. b) two primers, complementary to each end of the target sequence. c) a large supply of DNA nucleotides. d) a heat-stable DNA polymerase. e) All of the above
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
DNA from 100 unrelated people was amplified by PCR with allele specific primers, The PCR products were separated and visualized on the following gel. A specific fragment was found to correspond to each blood group allele, indicated on the left hand side. The number of individuals with each gel pattern is shown across the top. 7351-749, 18 Assuming random mating, what is the expected frequency of Type B blood? А 0.0025 0.070 C. 0.0725 D 0.25 E 0.05
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
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