You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...

Question

Question

You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...

You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution of the protein. How much of the stock and how much water should you use?

stock =

water =

science biology

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

Add a comment

Answer 2

Similar Homework Help Questions

You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...

You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below. Sample Protein Concentration mg/mL Protein Sample (microliter) Water (microliter) 4X Sample Buffer (microliter) Total Sample Volume (microliter) Total Lysate (-) 1 mg/mL 20 E1 0.07 mg/mL 20 E2 0.15 mg/mL 20 E3 0.2 mg/mL 20
If you have 14ml of a solution of 15% KOH and want to make 10ml of...

If you have 14ml of a solution of 15% KOH and want to make 10ml of a 9% solution, how would you prepare the solution? You would add ml of the 15% KOH to a graduated cylinder and add ml of water.Answers should be numerical (eg, 1, not one)
To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse...

To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse water to make up to 15ml . You have 4ml protein sample, calculate how many water and Lammeli Buffer you need to add? Laemmli final concentration should be 1x.

Calculations Needed: ANSWER ALL QUESTIONS IN ONE SINGLE POST PLEASE!!!! 1. Prepare 10ml of a 10%...

Calculations Needed: ANSWER ALL QUESTIONS IN ONE SINGLE POST PLEASE!!!! 1. Prepare 10ml of a 10% solution of Triton-x100 using undiluted Triton-100. Hint: Undiluted solution are always 100% I got 1mL for this question. I am not sure if I am right though. 2. You have 300μl of reaction for a PCR experiment. You want to adjust so that there is 1.25 U of Taq polymerase per 50μl. The taq is supplied as 5U/μl. How much Taq should you add...
In an experiment, you need to create 275 mL of a 1M solution. You have 2L...

In an experiment, you need to create 275 mL of a 1M solution. You have 2L of a 5M stock solution. Calculate how much stock solution you would need to mix with water to make a 1M solution.You would take mL of the 5M stock solution in a flask and add mL of distilled water.Answers should be numerical (eg, 1, not one)
How would you prepare a 2.5m solution of HNO3 from 10mL of 14m HNO3 ?

How would you prepare a 2.5m solution of HNO3 from 10mL of 14m HNO3 ?

4) You have a 50 mM stock solution of sodium dodecylsulfate and you need to make...

4) You have a 50 mM stock solution of sodium dodecylsulfate and you need to make 50 mL of a 0.1 mM solution. How much sodium dodecylsulfate will you need from the stock solution? 5) For Question #4, how much water will you need to add?
11)How do you prepare 30mm 10ml H2SO4 solution from 96%(w/w) and 1.84 g/ml stock H2SO4 solution? (H2SO4=98,08 g/mol)

11)How do you prepare 30mm 10ml H2SO4 solution from 96%(w/w) and 1.84 g/ml stock H2SO4 solution? (H2SO4=98,08 g/mol)
You need 1 liter of 1x TAE, you have a 5x stock solution. How much 5x...

You need 1 liter of 1x TAE, you have a 5x stock solution. How much 5x TAE stock do you need in ml? ml How much water do you need in ml? ml

Solution Preparation You need to prepare solutions for the reactions you will perform in the laboratory...

Solution Preparation You need to prepare solutions for the reactions you will perform in the laboratory experiment. You will be given a 0.02M stock solution of KMnO4 and a 0.5M stock solution of H2C2O4. The total volume of the solutions should always be 30 mL. You will use DI water to complete the volume as needed. Complete the table below and copy the results into your lab notebook for use during the lab: Run Volume KMnO4 (mL) Volume H2C2O4 (mL)...

You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...

Homework Answers

Add Answer to:
You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...

Post as a guest

Earn Coins

You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...

If you have 14ml of a solution of 15% KOH and want to make 10ml of...

To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse...

Calculations Needed: ANSWER ALL QUESTIONS IN ONE SINGLE POST PLEASE!!!! 1. Prepare 10ml of a 10%...

In an experiment, you need to create 275 mL of a 1M solution. You have 2L...

How would you prepare a 2.5m solution of HNO3 from 10mL of 14m HNO3 ?

4) You have a 50 mM stock solution of sodium dodecylsulfate and you need to make...

11)How do you prepare 30mm 10ml H2SO4 solution from 96%(w/w) and 1.84 g/ml stock H2SO4 solution? (H2SO4=98,08 g/mol)

You need 1 liter of 1x TAE, you have a 5x stock solution. How much 5x...

Solution Preparation You need to prepare solutions for the reactions you will perform in the laboratory...

You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...

Homework Answers

Add Answer to: You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...

Post as a guest

Earn Coins

Add Answer to:
You have a protein solution of 200uM. You need to prepare 10ml of a 200nM solution...