You need 1 liter of 1x TAE, you have a 5x stock solution. How much 5x...
To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
5) You have a 25X stock and need to make 1.5 L of 1X. How much of the 25X stock do you add and how much water? ruary
You are given a bottle containing 100 mL of a 50X TAE stock solution and are told to make 900 mL of 1X TAE solution. Describe what you would do.
how do you make 500ml of 1X SDS running buffer from a 5X stock solution?
2. Describe how you would produce 10 bottles of 750ml of 1x TAE from a stock of 50x TAE. Include only necessary calculations and steps that should be taken.
You must dilute a 5X stock solution of a hydrogen peroxide to 500 mL to dispense a 5 % solution. a. How much of the 5X stock do you need? b.What is the concentration of the 5X stock solution?
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit. Info about stocks: Agarose stock is powder Gel Red concentrate is 25,000X liquid
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
4) You have a 50 mM stock solution of sodium dodecylsulfate and you need to make 50 mL of a 0.1 mM solution. How much sodium dodecylsulfate will you need from the stock solution? 5) For Question #4, how much water will you need to add?
You have a 50X stock of electrophoresis buffer and you need 150ul of 1X. How do you make this happen? For Vs i got 3ul of 50X stock needed to prepare 150 ul of 1X. Then I got 147ul of h2o needed to prep 150ul of 1X. I am wondering if these are correct and if I am using the right wording with my answers? Should i be wording them in a different way? just don't want to get marked...