You are given a bottle containing 100 mL of a 50X TAE stock solution and are told to make 900 mL of 1X TAE solution. Describe what you would do.
C1V1= C2V2
C1= concentration of the stock solution
V1 - the volume of stock solution that is used to make the working solution
C2 - concentration of the working solution.
V2 - Final volume
For the given solution
C1 = 50X
V1= need to find out.
V2 = 900ml
C2 = 1X
50 * V1 = 1 = 900
CV1 = 900/50 = 18.
So we need to add 18 ml from 50X TAE and 882 ml water to make 900 ml of 1X TAE from 50X stock TAE
You are given a bottle containing 100 mL of a 50X TAE stock solution and are...
To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
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