How would you serially dilute an overnight bacterial culture to 10-3 using only 1 to 10 dilutions?
How would you serially dilute an overnight bacterial culture to 10-3 using only 1 to 10...
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a series of 1 or 10 ml dilutions (maximum 1:100) to obtain a final 200 ml culture at 1x103 cells/ml? Show your work for each step
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
You have 5ml of an overnight culture (2x108 cells per ml). How would you prepare a series of 1 or 10ml dilutions (maximum dilution 1:100) to obtain a final 200 ml culture at 1x103 cell/ml? Provide work for each step.
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
To dilute a bacterial culture, 100 mu l of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. The 16 hour culture was diluted by a factor of: _____ An antibiotic (ampicillin) used during a Transformation experiment. Ampicillin is stored as a stock solution of 100 mg/ml. You are pouring plates from a 500 ml flask of Lauria Bertini agar (LB Agar) and you need an antibiotic concentration of 100 mu g/ml...
How would you priduce a 10^-1 dilution of a 3 mL bacterial sample using the entire 3mL volume?
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...