What are two differences between the core promoter and the regulatory promoter for RNA pol II of eukaryotes?
What are two differences between the core promoter and the regulatory promoter for RNA pol II...
What are the four common core promoter elements for eukaryotic genes transcribed by RNA polymerase II? Must all of these elements be present in the promoter for transcription of every gene to occur? Explain
Please include: RNA pol II, TBP, TFIIB, TFIID, TCF4. Mediator, BRE, TATA, Inr, DPE, E box Question 5: (5 marks) Mediator is a protein complex that is able to act as a bridge between transcriptional activators and the TFIID-dependent RNA Pol II machinery. Draw what you would expect the promoter region to look like with Mediator acting at the promoter of a gene with an E-box 3000bp upstream of the transcriptional start site, which requires TCF4 for activation of transcription....
State three characteristics of core promoter elements for RNA polymerase II transcribed genes
Describe the structure and function of elements needed for transcription, including the promoter, RNA polymerase core enzyme and holoenzyme, sigma factor, and template and non-template (coding) strands of DNA. eukaryotes - . List major differences between transcription and RNA processing in bacteria and o What is coupled transcription/translation? o What is a polyribosome? Is it exclusive of bacterz - Discuss major components and events in RNA processing, in - Describe tRNA stru - Discuss mech cluding, introns and exons, splicing....
Can someone please help me answer these questions. Thank you! Eukaryotic transcription signals a) This drawing shows the placements of the four main sequences of the eukaryotic core promoter for RNA polymerase II. Identify each one and give a brief explanation b) Which sequences are used in a DPE-driven promoter? c) Which ones are used in a TATA-driven promoter? d) Please draw and describe the steps as the transcription factors work with eukaryotic RNA polymerase II to start transcription of...
Which of these is involved in the transcription of tRNA genes? A. RNA Pol 1 B. RNA Pol 2 C.RNA Pol 3 D.RNA Pol 4 E. Not sure A promoter is.... A. Proteins that defines the beginning of a gene B. DNA elements that help define the beginning of a gene C. It is the DNA sequence at the start of a gene D. Not sure Promoters TATA Start site What is the TATA box? A. It's a DNA sequence...
What are transcription factors? regulatory DNA sequences that bind to the promoter region of a gene regulatory DNA sequences that bind to a protein regulatory motifs that bind to the promoter region of a gene regulatory proteins that bind to specific DNA sequences
After performing ChIP-seq, you find that the protein you are working on binds to the promoter and just into the gene body of many genes throughout the genome. You hypothesize this protein may be important for RNA Pol II pausing and you want to gain nucleotide resolution of the transcripts this protein directly regulates. After depleting this protein, what technique would you perform to determine, at nucleotide resolution, the transcription and potential change to RNA Pol II pausing resulting loss...
F) The transcription initiation site is located (3 pts) i) within the promoter. ii) on RNA polymerase. iii) at splice sites. iv) in a ribosome. v) all of the above
son Label the diagram below (A-Protein coding region B-Regulatory switches C-Promoter D. mRNA e. RNA polymerase) (2) f. Assume that a fish inherits a deletion mutation in the pituitary switch such that the switch becomes inactive. You isolate DNA from jaw, pelvic, eye, and pituitary tissues. In the DNA of which tissue(s) would you expect the pituitary switch mutation? (2)