Why is de-ionized (ion-exchange) water not suitable as a solvent for use in UV absorbance measurements?
Why is de-ionized (ion-exchange) water not suitable as a solvent for use in UV absorbance measurements?
Homework Answers
Answer
De-ionized water is described as the water that lacks ions like sodium, calcium etc. The ionic impurity is removed in Deionised water hence it has low conductivity.
In the case of contamination of plasticizers or organic impurity, the water quality will be degraded although the conductivity will not rise. the total carbon content in deionized water will be high. Measurement of water conductivity for quality check of deionised will be meaningless. The pure water will have low conductivity and TOC hence MilliQ is recommended as a solvent for UV absorbance measurement. It also has low contamination than deionized water and will not show any UV absorbance peak.
As high UV absorbance is observed in deionized water due to its conductivity, it is not suitable as a solvent for use in UV absorbance measurement.
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