1. When running a western blot, what is the purpose of the marker? 2. A Western...
1. When running a western blot, what is the purpose of the marker?
2. A Western blot separates proteins based on _________.
Homework Answers
1. A protein marker is using for the estimation of the size of proteins resolved by gel electrophoresis.The purpose of the marker when running a western blot is that markers are used here to monitor transfer efficiency from gel to the blotting membrane.
2.By 3D structure or denatured proteins
Add Answer to:
1. When running a western blot, what is the purpose of the
marker?
2. A Western...
1) What does the appearance of a new band in a western blot mean? 2) When...
1) What does the appearance of a new band in a western blot mean? 2) When a band on a western blot increases in density over time, what does it mean? 3) When a band on a western blot decreases in density over time, what does it mean? 4) As it relates to EGF signaling, what process in the cell would possibly lead a band to increase in density over time? 5) As it relates to EGF signaling, what process...
100 1. How western blot does works and explain some of its possible application? 2. While...
100 1. How western blot does works and explain some of its possible application? 2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why was the SDS necessary for running the gel? 3. Why was it necessary for the transfer to the nitrocellulose membrane? 4. The first stage of western blotting involves separating proteins using gel electrophoresis. On what basis does this technique separate proteins? 5. How can you test the efficiency of transfer of the protein...
When developing a western blot what two factors influence the intensity of the band?
When developing a western blot what two factors influence the intensity of the band?
1. What is the major difference between western blot and Immunohistochemistry? 2. What is the difference...
1. What is the major difference between western blot and Immunohistochemistry? 2. What is the difference between a monoclonal and polyclonal antibody? 3. When is antigen retrieval necessary? What is the developing reagent used for immunohistochemistry in this weeks practice? How this reagent different from the substrates used in ELISpots and ELISA assays? 4.
Western Blot of LDH: 1. Suppose, upon developing your blot, you see more bands that you...
Western Blot of LDH: 1. Suppose, upon developing your blot, you see more bands that you expect. What are two possible reasons for this? 2. Suppose, upon developing your blot, you see no bands at all. What are two possible reasons for this?
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The...
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The images above show a northern blot using a probe to identify the let-7 miRNA (left), and a western blot (right) with a primary antibody to recognize Lin-41, a let-7 target. Assume each lane in both the northern and western blots come from the same sample. Which lane best matches each scenario described listed below? Normally processed let-7 miRNA let-7 miRNA in a Drosha mutant...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique such as procedure, reagents, its usage and applications. (5points) 2. Discuss common features of Random Probe Labeling share with RT-PCR. What are major differences between these two techniques? (5points) 3. It is claimed by a pharmaceutical company that protein A' is beneficial to treat breast cancer since it promotes apoptosis. If you were put into charge of testing this, how would you design experiment?...
Think about the two methods (the western and dot blot) how they differ, what unique benefits...
Think about the two methods (the western and dot blot) how they differ, what unique benefits might each technique present? When might one be more useful than the other?
For each of the following, what are you running on the gel, and what is being...
For each of the following, what are you running on the gel, and what is being used as the probe? (DNA, RNA, Protein, or Antibodies) Gel Probe 28) Southern blot = 29) Western blot 30) Northern blot =
Picture is at bottom. The figure of the western blot. Figure legend is also below that....
Picture is at bottom. The figure of the western blot. Figure
legend is also below that.
Question 7. (4 pt) The data shown in the figure below is from a study of the regulation of a protein called YB-1. a.) Based on the data in the figure, what mechanism of regulation is most likely induced by the treatments? b.) Briefly explain the evidence in the data that supports your conclusion about the mechanism. c.) What is the loading control in...
100 1. How western blot does works and explain some of its possible application? 2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why was the SDS necessary for running the gel? 3. Why was it necessary for the transfer to the nitrocellulose membrane? 4. The first stage of western blotting involves separating proteins using gel electrophoresis. On what basis does this technique separate proteins? 5. How can you test the efficiency of transfer of the protein...
1. What is the major difference between western blot and Immunohistochemistry? 2. What is the difference between a monoclonal and polyclonal antibody? 3. When is antigen retrieval necessary? What is the developing reagent used for immunohistochemistry in this weeks practice? How this reagent different from the substrates used in ELISpots and ELISA assays? 4.
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The images above show a northern blot using a probe to identify the let-7 miRNA (left), and a western blot (right) with a primary antibody to recognize Lin-41, a let-7 target. Assume each lane in both the northern and western blots come from the same sample. Which lane best matches each scenario described listed below? Normally processed let-7 miRNA let-7 miRNA in a Drosha mutant...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique such as procedure, reagents, its usage and applications. (5points) 2. Discuss common features of Random Probe Labeling share with RT-PCR. What are major differences between these two techniques? (5points) 3. It is claimed by a pharmaceutical company that protein A' is beneficial to treat breast cancer since it promotes apoptosis. If you were put into charge of testing this, how would you design experiment?...
For each of the following, what are you running on the gel, and what is being used as the probe? (DNA, RNA, Protein, or Antibodies) Gel Probe 28) Southern blot = 29) Western blot 30) Northern blot =
Picture is at bottom. The figure of the western blot. Figure
legend is also below that.
Question 7. (4 pt) The data shown in the figure below is from a study of the regulation of a protein called YB-1. a.) Based on the data in the figure, what mechanism of regulation is most likely induced by the treatments? b.) Briefly explain the evidence in the data that supports your conclusion about the mechanism. c.) What is the loading control in...
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